| RRC ID |
53407
|
| 著者 |
Zhou X, Feng X, Mao H, Li M, Xu F, Hu K, Guang S.
|
| タイトル |
RdRP-synthesized antisense ribosomal siRNAs silence pre-rRNA via the nuclear RNAi pathway.
|
| ジャーナル |
Nat Struct Mol Biol
|
| Abstract |
Expression of rRNA affects cell growth and proliferation, but mechanisms that modulate rRNA levels are poorly understood. We conducted a genetic screen for factors that negatively regulate generation of endogenous short interfering RNA (endo-siRNA) in Caenorhabditis elegans and identified a suppressor of siRNA (susi-1) and antisense ribosomal siRNAs (risiRNAs). risiRNAs show sequence complementary to 18S and 26S rRNAs and require RNA-dependent RNA polymerases (RdRPs) for their production. They act through the nuclear RNA interference (RNAi) pathway to downregulate pre-rRNA. Stress stimuli, including low temperature and UV irradiation, induced the accumulation of risiRNAs. SUSI-1 is a homolog of the human DIS3L2 exonuclease involved in 3'-5' degradation of oligouridylated RNAs. In susi-1 mutant and in low temperature-treated animals, 3'-tail oligouridylated 26S rRNA accumulated. The injection of oligouridylated rRNA elicited nuclear accumulation of NRDE-3. Our findings identify a new subset of 22G-RNAs that regulate pre-rRNA expression and a mechanism to maintain rRNA homeostasis.
|
| 巻・号 |
24(3)
|
| ページ |
258-269
|
| 公開日 |
2017-3-1
|
| DOI |
10.1038/nsmb.3376
|
| PII |
nsmb.3376
|
| PMID |
28165511
|
| MeSH |
Amino Acid Sequence
Animals
Caenorhabditis elegans / metabolism
Caenorhabditis elegans / radiation effects
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / metabolism
Cell Nucleus / metabolism*
Cell Nucleus / radiation effects
Down-Regulation / genetics
Down-Regulation / radiation effects
Green Fluorescent Proteins / metabolism
High-Throughput Nucleotide Sequencing
Models, Biological
Mutation / genetics
RNA Interference*
RNA Precursors / genetics
RNA Precursors / metabolism*
RNA Stability / radiation effects
RNA, Antisense / genetics*
RNA, Antisense / metabolism
RNA, Ribosomal / genetics
RNA, Ribosomal / metabolism*
RNA, Small Interfering / metabolism*
RNA-Dependent RNA Polymerase / metabolism*
Ribosomes / genetics
Ribosomes / metabolism*
Sequence Analysis, RNA
Sequence Homology, Amino Acid
Subcellular Fractions / metabolism
Subcellular Fractions / radiation effects
Temperature
Ultraviolet Rays
Uridine / metabolism
|
| IF |
11.98
|
| 引用数 |
13
|
| リソース情報 |
| 線虫 |
tm1200 |
