RRC ID 53405
著者 Jang H, Levy S, Flavell SW, Mende F, Latham R, Zimmer M, Bargmann CI.
タイトル Dissection of neuronal gap junction circuits that regulate social behavior in Caenorhabditis elegans.
ジャーナル Proc Natl Acad Sci U S A
Abstract A hub-and-spoke circuit of neurons connected by gap junctions controls aggregation behavior and related behavioral responses to oxygen, pheromones, and food in Caenorhabditis elegans The molecular composition of the gap junctions connecting RMG hub neurons with sensory spoke neurons is unknown. We show here that the innexin gene unc-9 is required in RMG hub neurons to drive aggregation and related behaviors, indicating that UNC-9-containing gap junctions mediate RMG signaling. To dissect the circuit in detail, we developed methods to inhibit unc-9-based gap junctions with dominant-negative unc-1 transgenes. unc-1(dn) alters a stomatin-like protein that regulates unc-9 electrical signaling; its disruptive effects can be rescued by a constitutively active UNC-9::GFP protein, demonstrating specificity. Expression of unc-1(dn) in RMG hub neurons, ADL or ASK pheromone-sensing neurons, or URX oxygen-sensing neurons disrupts specific elements of aggregation-related behaviors. In ADL, unc-1(dn) has effects opposite to those of tetanus toxin light chain, separating the roles of ADL electrical and chemical synapses. These results reveal roles of gap junctions in a complex behavior at cellular resolution and provide a tool for similar exploration of other gap junction circuits.
巻・号 114(7)
ページ E1263-E1272
公開日 2017-2-14
DOI 10.1073/pnas.1621274114
PII 1621274114
PMID 28143932
PMC PMC5320989
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Electrical Synapses / genetics Electrical Synapses / metabolism* Gap Junctions / genetics Gap Junctions / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Motor Activity / genetics Pheromones / metabolism Sensory Receptor Cells / metabolism* Signal Transduction / genetics Social Behavior*
IF 9.412
引用数 14
リソース情報
線虫 tm3524 tm2738 tm3393 tm3394 tm1589 tm3829 tm1896 tm4591 tm1661