RRC ID |
53354
|
著者 |
Ujisawa T, Ohta A, Uda-Yagi M, Kuhara A.
|
タイトル |
Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.
|
ジャーナル |
PLoS One
|
Abstract |
Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron.
|
巻・号 |
11(10)
|
ページ |
e0165518
|
公開日 |
2016-1-1
|
DOI |
10.1371/journal.pone.0165518
|
PII |
PONE-D-16-19856
|
PMID |
27788246
|
PMC |
PMC5082853
|
MeSH |
Adaptation, Physiological
Animals
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans / physiology
Calcium / metabolism
Cold Temperature
GTP-Binding Proteins / metabolism*
Germ Cells
Microscopy, Confocal
Signal Transduction*
Temperature*
|
IF |
2.74
|
引用数 |
5
|
リソース情報 |
線虫 |
tm3098 |