RRC ID 53346
著者 Yogev S, Cooper R, Fetter R, Horowitz M, Shen K.
タイトル Microtubule Organization Determines Axonal Transport Dynamics.
ジャーナル Neuron
Abstract Axonal microtubule (MT) arrays are the major cytoskeleton substrate for cargo transport. How MT organization, i.e., polymer length, number, and minus-end spacing, is regulated and how it impinges on axonal transport are unclear. We describe a method for analyzing neuronal MT organization using light microscopy. This method circumvents the need for electron microscopy reconstructions and is compatible with live imaging of cargo transport and MT dynamics. Examination of a C. elegans motor neuron revealed how age, MT-associated proteins, and signaling pathways control MT length, minus-end spacing, and coverage. In turn, MT organization determines axonal transport progression: cargoes pause at polymer termini, suggesting that switching MT tracks is rate limiting for efficient transport. Cargo run length is set by MT length, and higher MT coverage correlates with shorter pauses. These results uncover the principles and mechanisms of neuronal MT organization and its regulation of axonal cargo transport.
巻・号 92(2)
ページ 449-460
公開日 2016-10-19
DOI 10.1016/j.neuron.2016.09.036
PII S0896-6273(16)30637-7
PMID 27764672
PMC PMC5432135
MeSH Animals Axonal Transport* Caenorhabditis elegans Cytoskeleton / metabolism* Dyneins / metabolism Kinesins / metabolism Microscopy Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Microtubules / ultrastructure Motor Neurons / metabolism* Motor Neurons / ultrastructure Polymers / metabolism* Signal Transduction Time-Lapse Imaging
IF 14.415
引用数 42
リソース情報
線虫 tm1357 tm4830 tm2143 tm3737 tm3124 tm5775