RRC ID 53292
著者 Imanikia S, Galea F, Nagy E, Phillips DH, Stürzenbaum SR, Arlt VM.
タイトル The application of the comet assay to assess the genotoxicity of environmental pollutants in the nematode Caenorhabditis elegans.
ジャーナル Environ Toxicol Pharmacol
Abstract This study aimed to establish a protocol for cell dissociation from the nematode Caenorhabditis elegans (C. elegans) to assess the genotoxicity of the environmental pollutant benzo[a]pyrene (BaP) using the alkaline version of the single cell electrophoresis assay (comet assay). BaP genotoxicity was assessed in C. elegans (wild-type [WT]; N2, Bristol) after 48h exposure (0-40μM). Induction of comets by BaP was concentration-dependent up to 20μM; comet% tail DNA was ∼30% at 20μM BaP and ∼10% in controls. Similarly, BaP-induced DNA damage was evaluated in C. elegans mutant strains deficient in DNA repair. In xpa-1 and apn-1 mutants BaP-induced comet formation was diminished to WT background levels suggesting that the damage formed by BaP that is detected in the comet assay is not recognised in cells deficient in nucleotide and base excision repair, respectively. In summary, our study provides a protocol to evaluate DNA damage of environmental pollutants in whole nematodes using the comet assay.
巻・号 45
ページ 356-61
公開日 2016-7-1
DOI 10.1016/j.etap.2016.06.020
PII S1382-6689(16)30158-2
PMID 27389785
PMC PMC4962771
MeSH Animals Benzo(a)pyrene / toxicity* Caenorhabditis elegans / drug effects* Caenorhabditis elegans / genetics Comet Assay / methods* DNA Damage* Dose-Response Relationship, Drug Environmental Monitoring / methods* Environmental Pollutants / toxicity* Mutagens / toxicity*
IF 3.292
引用数 12
リソース情報
線虫 tm2842