| RRC ID |
53240
|
| 著者 |
Chen JX, Cipriani PG, Mecenas D, Polanowska J, Piano F, Gunsalus KC, Selbach M.
|
| タイトル |
In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics.
|
| ジャーナル |
Mol Cell Proteomics
|
| Abstract |
Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.
|
| 巻・号 |
15(5)
|
| ページ |
1642-57
|
| 公開日 |
2016-5-1
|
| DOI |
10.1074/mcp.M115.053975
|
| PII |
S1535-9476(20)33603-3
|
| PMID |
26912668
|
| PMC |
PMC4858945
|
| MeSH |
Animals
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / analysis*
Caenorhabditis elegans Proteins / metabolism
Carrier Proteins / metabolism*
Chromatography, Affinity
Cytoplasmic Granules / metabolism
Gene Expression Regulation, Developmental
Mass Spectrometry
Phosphorylation
Protein Interaction Maps
Protein Serine-Threonine Kinases / metabolism*
Protein-Tyrosine Kinases / metabolism*
Proteomics / methods*
|
| IF |
4.87
|
| 引用数 |
10
|
| リソース情報 |
| 線虫 |
tm4526
tm4259
tm4343
tm3103 |
