RRC ID 51557
著者 Yue X, Zhao J, Li X, Fan Y, Duan D, Zhang X, Zou W, Sheng Y, Zhang T, Yang Q, Luo J, Duan S, Xiao R, Kang L.
タイトル TMC Proteins Modulate Egg Laying and Membrane Excitability through a Background Leak Conductance in C. elegans.
ジャーナル Neuron
Abstract Membrane excitability is a fundamentally important feature for all excitable cells including both neurons and muscle cells. However, the background depolarizing conductances in excitable cells, especially in muscle cells, are not well characterized. Although mutations in transmembrane channel-like (TMC) proteins TMC1 and TMC2 cause deafness and vestibular defects in mammals, their precise action modes are elusive. Here, we discover that both TMC-1 and TMC-2 are required for normal egg laying in C. elegans. Mutations in these TMC proteins cause membrane hyperpolarization and disrupt the rhythmic calcium activities in both neurons and muscles involved in egg laying. Mechanistically, TMC proteins enhance membrane depolarization through background leak currents and ectopic expression of both C. elegans and mammalian TMC proteins results in membrane depolarization. Therefore, we have identified an unexpected role of TMC proteins in modulating membrane excitability. Our results may provide mechanistic insights into the functions of TMC proteins in hearing loss and other diseases.
巻・号 97(3)
ページ 571-585.e5
公開日 2018-2-7
DOI 10.1016/j.neuron.2017.12.041
PII S0896-6273(17)31209-6
PMID 29395910
PMC PMC7038793
MeSH Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Ion Channels / metabolism Ion Channels / physiology* Membrane Potentials* Muscles / physiology* Neurons / metabolism Neurons / physiology* Sexual Behavior, Animal*
IF 14.415
引用数 13
リソース情報
線虫 tm1851