| RRC ID |
51541
|
| 著者 |
Dejima K, Hori S, Iwata S, Suehiro Y, Yoshina S, Motohashi T, Mitani S.
|
| タイトル |
An Aneuploidy-Free and Structurally Defined Balancer Chromosome Toolkit for Caenorhabditis elegans.
|
| ジャーナル |
Cell Rep
|
| Abstract |
Balancer chromosomes are critical tools for genetic research. In C. elegans, reciprocal translocations that lead to aneuploidy have been widely used to maintain lethal and sterile mutations in stable stocks. Here, we generated a set of aneuploidy-free and structurally defined crossover suppressors that contain two overlapping inversions using the CRISPR/Cas9 system. The toolkit includes 13 crossover suppressors and covers approximately 63% of all C. elegans coding genes. Together with the classical intrachromosomal crossover suppressors, the system now covers 89% of the coding genes. We also labeled the created balancers with fluorescent and phenotypic markers. We show that the crossover suppressors are better for embryonic analysis compared with translocational balancers. Additionally, we demonstrate an efficient method to generate lethal alleles by targeting essential genes on a chromosome balanced with a crossover suppressor. The toolkit will allow more efficient experiments in which lethal and sterile mutants can be analyzed.
|
| 巻・号 |
22(1)
|
| ページ |
232-241
|
| 公開日 |
2018-1-2
|
| DOI |
10.1016/j.celrep.2017.12.024
|
| PII |
S2211-1247(17)31833-8
|
| PMID |
29298424
|
| MeSH |
Aneuploidy
Animals
CRISPR-Cas Systems*
Caenorhabditis elegans / genetics*
Chromosomes / genetics*
|
| IF |
8.109
|
| 引用数 |
14
|
| リソース情報 |
| 線虫 |
tm1794
tm1986
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tm7168
tm750
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tm9723 |
