論文 - 詳細
| RRC ID | 51536 |
|---|---|
| 著者 | Sato M, Sato K, Tomura K, Kosako H, Sato K. |
| タイトル | The autophagy receptor ALLO-1 and the IKKE-1 kinase control clearance of paternal mitochondria in Caenorhabditis elegans. |
| ジャーナル | Nat Cell Biol |
| Abstract |
In Caenorhabditis elegans embryos, paternally provided organelles, including mitochondria, are eliminated by a process of selective autophagy called allophagy, the mechanism by which mitochondrial DNA is inherited maternally. However, it remains unclear how paternal organelles are recognized and targeted for autophagy. Here, we identified an autophagy receptor for allophagy, ALLO-1. ALLO-1 is essential for autophagosome formation around paternal organelles and directly binds to the worm LC3 homologue LGG-1 through its LC3-interacting region (LIR) motif. After fertilization, ALLO-1 accumulates on the paternal organelles before autophagosome formation, and this localization depends on the ubiquitin modification of the paternal organelles. We also identified IKKE-1, a worm homologue of the TBK1 and IKKε family kinase, as another critical regulator of allophagy. IKKE-1 interacts with ALLO-1, and the IKKE-1-dependent phosphorylation of ALLO-1 is important for paternal organelle clearance. Thus, we propose that ALLO-1 is the allophagy receptor whose function is regulated by IKKE-1-dependent phosphorylation. |
| 巻・号 | 20(1) |
| ページ | 81-91 |
| 公開日 | 2018-1-1 |
| DOI | 10.1038/s41556-017-0008-9 |
| PII | 10.1038/s41556-017-0008-9 |
| PMID | 29255173 |
| MeSH | Animals Autophagosomes Autophagy / genetics* Autophagy-Related Proteins / genetics* Autophagy-Related Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism DNA, Mitochondrial / genetics DNA, Mitochondrial / metabolism Embryo, Nonmammalian Female Gene Expression Regulation Inheritance Patterns* Male Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism Mitochondria / metabolism* Phosphorylation Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism Signal Transduction Ubiquitin / genetics Ubiquitin / metabolism Ubiquitination |
| IF | 20.042 |
| 引用数 | 12 |
| リソース情報 | |
| 線虫 | tm2508 tm4012 tm4102 tm4756 |