RRC ID 51524
著者 Lee JS, Shih PY, Schaedel ON, Quintero-Cadena P, Rogers AK, Sternberg PW.
タイトル FMRFamide-like peptides expand the behavioral repertoire of a densely connected nervous system.
ジャーナル Proc Natl Acad Sci U S A
Abstract Animals, including humans, can adapt to environmental stress through phenotypic plasticity. The free-living nematode Caenorhabditis elegans can adapt to harsh environments by undergoing a whole-animal change, involving exiting reproductive development and entering the stress-resistant dauer larval stage. The dauer is a dispersal stage with dauer-specific behaviors for finding and stowing onto carrier animals, but how dauers acquire these behaviors, despite having a physically limited nervous system of 302 neurons, is poorly understood. We compared dauer and reproductive development using whole-animal RNA sequencing at fine time points and at sufficient depth to measure transcriptional changes within single cells. We detected 8,042 genes differentially expressed during dauer and reproductive development and observed striking up-regulation of neuropeptide genes during dauer entry. We knocked down neuropeptide processing using sbt-1 mutants and demonstrate that neuropeptide signaling promotes the decision to enter dauer rather than reproductive development. We also demonstrate that during dauer neuropeptides modulate the dauer-specific nictation behavior (carrier animal-hitchhiking) and are necessary for switching from repulsion to CO2 (a carrier animal cue) in nondauers to CO2 attraction in dauers. We tested individual neuropeptides using CRISPR knockouts and existing strains and demonstrate that the combined effects of flp-10 and flp-17 mimic the effects of sbt-1 on nictation and CO2 attraction. Through meta-analysis, we discovered similar up-regulation of neuropeptides in the dauer-like infective juveniles of diverse parasitic nematodes, suggesting the antiparasitic target potential of SBT-1. Our findings reveal that, under stress, increased neuropeptide signaling in C. elegans enhances their decision-making accuracy and expands their behavioral repertoire.
巻・号 114(50)
ページ E10726-E10735
公開日 2017-12-12
DOI 10.1073/pnas.1710374114
PII 1710374114
PMID 29167374
PMC PMC5740649
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / physiology* Carbon Dioxide / metabolism Clustered Regularly Interspaced Short Palindromic Repeats FMRFamide / chemistry Gene Knockdown Techniques Genes, Helminth Larva / growth & development Larva / physiology Neuropeptides / genetics Neuropeptides / physiology* Phenotype RNA, Helminth Reproduction Sequence Analysis, RNA Signal Transduction Stress, Physiological
IF 9.412
引用数 12
リソース情報
線虫 tm2427 tm2706