RRC ID 51519
著者 Lee CS, Lu T, Seydoux G.
タイトル Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B.
ジャーナル Elife
Abstract Nanos RNA-binding proteins are required for germline development in metazoans, but the underlying mechanisms remain poorly understood. We have profiled the transcriptome of primordial germ cells (PGCs) lacking the nanos homologs nos-1 and nos-2 in C. elegans. nos-1nos-2 PGCs fail to silence hundreds of transcripts normally expressed in oocytes. We find that this misregulation is due to both delayed turnover of maternal transcripts and inappropriate transcriptional activation. The latter appears to be an indirect consequence of delayed turnover of the maternally-inherited transcription factor LIN-15B, a synMuvB class transcription factor known to antagonize PRC2 activity. PRC2 is required for chromatin reprogramming in the germline, and the transcriptome of PGCs lacking PRC2 resembles that of nos-1nos-2 PGCs. Loss of maternal LIN-15B restores fertility to nos-1nos-2 mutants. These findings suggest that Nanos promotes germ cell fate by downregulating maternal RNAs and proteins that would otherwise interfere with PRC2-dependent reprogramming of PGC chromatin.
巻・号 6
公開日 2017-11-7
DOI 10.7554/eLife.30201
PII 30201
PMID 29111977
PMC PMC5734877
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / biosynthesis* Caenorhabditis elegans Proteins / metabolism* Down-Regulation* Epigenesis, Genetic* Gene Expression Profiling Germ Cells / physiology* Transcription Factors / biosynthesis*
IF 7.08
引用数 8
リソース情報
線虫 tm1489