RRC ID 51491
著者 Deffit SN, Yee BA, Manning AC, Rajendren S, Vadlamani P, Wheeler EC, Domissy A, Washburn MC, Yeo GW, Hundley HA.
タイトル The C. elegans neural editome reveals an ADAR target mRNA required for proper chemotaxis.
ジャーナル Elife
Abstract ADAR proteins alter gene expression both by catalyzing adenosine (A) to inosine (I) RNA editing and binding to regulatory elements in target RNAs. Loss of ADARs affects neuronal function in all animals studied to date. Caenorhabditis elegans lacking ADARs exhibit reduced chemotaxis, but the targets responsible for this phenotype remain unknown. To identify critical neural ADAR targets in C. elegans, we performed an unbiased assessment of the effects of ADR-2, the only A-to-I editing enzyme in C. elegans, on the neural transcriptome. Development and implementation of publicly available software, SAILOR, identified 7361 A-to-I editing events across the neural transcriptome. Intersecting the neural editome with adr-2 associated gene expression changes, revealed an edited mRNA, clec-41, whose neural expression is dependent on deamination. Restoring clec-41 expression in adr-2 deficient neural cells rescued the chemotaxis defect, providing the first evidence that neuronal phenotypes of ADAR mutants can be caused by altered gene expression.
巻・号 6
公開日 2017-9-19
DOI 10.7554/eLife.28625
PII 28625
PMID 28925356
PMC PMC5644944
MeSH Adenosine / metabolism Adenosine Deaminase / metabolism* Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Chemotaxis* Gene Expression Profiling Gene Expression Regulation* Inosine / metabolism RNA, Messenger / metabolism*
IF 7.08
引用数 12
リソース情報
線虫 tm668