論文 - 詳細
| RRC ID | 51485 |
|---|---|
| 著者 | Zilberman Y, Abrams J, Anderson DC, Nance J. |
| タイトル | Cdc42 regulates junctional actin but not cell polarization in the Caenorhabditis elegans epidermis. |
| ジャーナル | J Cell Biol |
| Abstract |
During morphogenesis, adherens junctions (AJs) remodel to allow changes in cell shape and position while preserving adhesion. Here, we examine the function of Rho guanosine triphosphatase CDC-42 in AJ formation and regulation during Caenorhabditis elegans embryo elongation, a process driven by asymmetric epidermal cell shape changes. cdc-42 mutant embryos arrest during elongation with epidermal ruptures. Unexpectedly, we find using time-lapse fluorescence imaging that cdc-42 is not required for epidermal cell polarization or junction assembly, but rather is needed for proper junctional actin regulation during elongation. We show that the RhoGAP PAC-1/ARHGAP21 inhibits CDC-42 activity at AJs, and loss of PAC-1 or the interacting linker protein PICC-1/CCDC85A-C blocks elongation in embryos with compromised AJ function. pac-1 embryos exhibit dynamic accumulations of junctional F-actin and an increase in AJ protein levels. Our findings identify a previously unrecognized molecular mechanism for inhibiting junctional CDC-42 to control actin organization and AJ protein levels during epithelial morphogenesis. |
| 巻・号 | 216(11) |
| ページ | 3729-3744 |
| 公開日 | 2017-11-6 |
| DOI | 10.1083/jcb.201611061 |
| PII | jcb.201611061 |
| PMID | 28903999 |
| PMC | PMC5674880 |
| MeSH | Actins / metabolism* Adherens Junctions / enzymology* Animals Animals, Genetically Modified Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Polarity* Embryo, Nonmammalian / enzymology Epidermis / embryology Epidermis / enzymology* Epithelial Cells / enzymology* GTP-Binding Proteins / genetics GTP-Binding Proteins / metabolism* GTPase-Activating Proteins / genetics GTPase-Activating Proteins / metabolism Gene Expression Regulation, Developmental Genotype Morphogenesis Mutation Phenotype Signal Transduction Time Factors |
| IF | 8.811 |
| 引用数 | 13 |
| リソース情報 | |
| 線虫 | tm1425 |