RRC ID 51455
著者 Noma K, Goncharov A, Ellisman MH, Jin Y.
タイトル Microtubule-dependent ribosome localization in C. elegans neurons.
ジャーナル Elife
Abstract Subcellular localization of ribosomes defines the location and capacity for protein synthesis. Methods for in vivo visualizing ribosomes in multicellular organisms are desirable in mechanistic investigations of the cell biology of ribosome dynamics. Here, we developed an approach using split GFP for tissue-specific visualization of ribosomes in Caenorhabditis elegans. Labeled ribosomes are detected as fluorescent puncta in the axons and synaptic terminals of specific neuron types, correlating with ribosome distribution at the ultrastructural level. We found that axonal ribosomes change localization during neuronal development and after axonal injury. By examining mutants affecting axonal trafficking and performing a forward genetic screen, we showed that the microtubule cytoskeleton and the JIP3 protein UNC-16 exert distinct effects on localization of axonal and somatic ribosomes. Our data demonstrate the utility of tissue-specific visualization of ribosomes in vivo, and provide insight into the mechanisms of active regulation of ribosome localization in neurons.
巻・号 6
公開日 2017-8-2
DOI 10.7554/eLife.26376
PII 26376
PMID 28767038
PMC PMC5577916
MeSH Adaptor Proteins, Signal Transducing / metabolism Animals Biological Transport Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Microtubules / metabolism* Neurons / physiology* Ribosomes / metabolism* Staining and Labeling
IF 7.08
引用数 12
リソース情報
線虫 tm3555