論文 - 詳細
| RRC ID | 51422 |
|---|---|
| 著者 | Brown KC, Svendsen JM, Tucci RM, Montgomery BE, Montgomery TA. |
| タイトル | ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Caenorhabditis elegans contains 25 Argonautes, of which, ALG-1 and ALG-2 are known to primarily interact with miRNAs. ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role in small RNA pathways is unknown. We analyzed by high-throughput sequencing the small RNAs associated with ALG-5, ALG-1 and ALG-2, as well as changes in mRNA expression in alg-5, alg-1 and alg-2 mutants. We show that ALG-5 defines a distinct branch of the miRNA pathway affecting the expression of genes involved in immunity, defense, and development. In contrast to ALG-1 and ALG-2, which associate with most miRNAs and have general roles throughout development, ALG-5 interacts with only a small subset of miRNAs and is specifically expressed in the germline where it localizes alongside the piRNA and siRNA machinery at P granules. alg-5 is required for optimal fertility and mutations in alg-5 lead to a precocious transition from spermatogenesis to oogenesis. Our results provide a near-comprehensive analysis of miRNA-Argonaute interactions in C. elegans and reveal a new role for miRNAs in the germline. |
| 巻・号 | 45(15) |
| ページ | 9093-9107 |
| 公開日 | 2017-9-6 |
| DOI | 10.1093/nar/gkx536 |
| PII | 3883740 |
| PMID | 28645154 |
| PMC | PMC5587817 |
| MeSH | Animals Argonaute Proteins / genetics* Argonaute Proteins / metabolism Caenorhabditis elegans / classification Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Gene Expression Regulation, Developmental* Germ Cells / growth & development Germ Cells / metabolism* Hermaphroditic Organisms / genetics Hermaphroditic Organisms / growth & development Hermaphroditic Organisms / metabolism High-Throughput Nucleotide Sequencing MicroRNAs / genetics MicroRNAs / metabolism Mutation Oogenesis / genetics Phylogeny Protein Isoforms / genetics Protein Isoforms / metabolism RNA, Helminth / genetics* RNA, Helminth / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism RNA-Binding Proteins / genetics* RNA-Binding Proteins / metabolism Spermatogenesis / genetics |
| IF | 11.502 |
| 引用数 | 12 |
| リソース情報 | |
| 線虫 | tm1163 |