RRC ID 51353
著者 Romanelli-Cedrez L, Carrera I, Otero L, Miranda-Vizuete A, Mariotti M, Alkema MJ, Salinas G.
タイトル Selenoprotein T is required for pathogenic bacteria avoidance in Caenorhabditis elegans.
ジャーナル Free Radic Biol Med
Abstract Selenoprotein T (SELENOT) is an endoplasmatic reticulum (ER)-associated redoxin that contains the amino acid selenocysteine (Sec, U) within a CXXU motif within a thioredoxin-like fold. Its precise function in multicellular organisms is not completely understood although it has been shown in mammals to be involved in Ca2+ homeostasis, antioxidant and neuroendocrine functions. Here, we use the model organism C. elegans to address SELENOT function in a whole organism throughout its life cycle. C. elegans possess two genes encoding SELENOT protein orthologues (SELT-1.1 and SELT-1.2), which lack Sec and contain the CXXC redox motif instead. Our results show that a Sec→Cys replacement and a gene duplication were two major evolutionary events that occurred in the nematode lineage. We find that worm SELT-1.1 localizes to the ER and is expressed in different cell types, including the nervous system. In contrast, SELT-1.2 exclusively localizes in the cytoplasm of the AWB neurons. We find that selt-1.1 and selt-1.2 single mutants as well as the double mutant are viable, but the selt-1.1 mutant is compromised under rotenone-induced oxidative stress. We demonstrate that selt-1.1, but not selt-1.2, is required for avoidance to the bacterial pathogens Serratia marcescens and Pseudomonas aeruginosa. Aversion to the noxious signal 2-nonanone is also significantly impaired in selt-1.1, but not in selt-1.2 mutant animals. Our results suggest that selt-1.1 would be a redox transducer required for nociception and optimal organismal fitness. The results highlight C. elegans as a valuable model organism to study SELENOT-dependent processes.
巻・号 108
ページ 174-182
公開日 2017-7-1
DOI 10.1016/j.freeradbiomed.2017.03.021
PII S0891-5849(17)30170-3
PMID 28347729
MeSH Animals Caenorhabditis elegans / immunology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cells, Cultured Cysteine / genetics Endoplasmic Reticulum / metabolism* Gene Duplication Immunity, Innate Ketones / administration & dosage Life Cycle Stages Mutation / genetics Neurons / metabolism* Nociception Oxidative Stress Protein Transport Pseudomonas Infections / immunology* Pseudomonas aeruginosa / immunology* Selenoproteins / genetics Selenoproteins / metabolism* Serratia Infections / immunology* Serratia marcescens / immunology*
IF 6.17
引用数 2
リソース情報
線虫 tm3763 tm3771