RRC ID |
50041
|
著者 |
Shan L, Wu C, Chen D, Hou L, Li X, Wang L, Chu X, Hou Y, Wang Z.
|
タイトル |
Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis.
|
ジャーナル |
J Genet Genomics
|
Abstract |
In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and is achieved by the RNA 3'-processing machinery to generate diverse 3'UTR profiles. In Drosophila spermatogenesis, we observed distinct profiles of transcriptome-wide 3'UTR between mitotic and meiotic cells. In mutant germ cells stuck in mitosis, 3'UTRs of hundreds of genes were consistently shifted. Remarkably, altering the levels of multiple 3'-processing factors disrupted germline's progression to meiosis, indicative of APA's active role in this transition. An RNA-binding protein (RBP) Tut could directly bind 3'UTRs of 3'-processing factors whose expressions were repressed in the presence of Tut-containing complex. Further, we demonstrated that this RBP complex could execute the repression post-transcriptionally by recruiting CCR4/Twin of deadenylation complex. Thus, we propose that an RBP complex regulates the dynamic APA profile to promote the mitosis-to-meiosis transition.
|
巻・号 |
44(2)
|
ページ |
95-106
|
公開日 |
2017-2-20
|
DOI |
10.1016/j.jgg.2016.12.007
|
PII |
S1673-8527(17)30017-6
|
PMID |
28190776
|
MeSH |
3' Untranslated Regions / genetics
Animals
Drosophila melanogaster / cytology
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism
Gene Expression Profiling
Male
Meiosis*
Mitosis*
Polyadenylation*
RNA-Binding Proteins / metabolism
|
IF |
5.065
|
引用数 |
3
|
リソース情報 |
ショウジョウバエ |
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