RRC ID 49340
著者 Kotoula V, Moressis A, Semelidou O, Skoulakis EMC.
タイトル Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in Drosophila.
ジャーナル Proc Natl Acad Sci U S A
Abstract Anesthesia-resistant memory (ARM) was described decades ago, but the mechanisms that underlie this protein synthesis-independent form of consolidated memory in Drosophila remain poorly understood. Whether the several signaling molecules, receptors, and synaptic proteins currently implicated in ARM operate in one or more pathways and how they function in the process remain unclear. We present evidence that Drk, the Drosophila ortholog of the adaptor protein Grb2, is essential for ARM within adult mushroom body neurons. Significantly, Drk signals engage the Rho kinase Drok, implicating dynamic cytoskeletal changes in ARM, and this is supported by reduced F-actin in the mutants and after pharmacological inhibition of Drok. Interestingly, Drk-Drok signaling appears independent of the function of Radish (Rsh), a protein long implicated in ARM, suggesting that the process involves at least two distinct molecular pathways. Based on these results, we propose that signaling pathways involved in structural plasticity likely underlie this form of translation-independent memory.
巻・号 114(41)
ページ 10984-10989
公開日 2017-10-10
DOI 10.1073/pnas.1704835114
PII 1704835114
PMID 28973902
PMC PMC5642687
MeSH Actins / metabolism* Anesthetics / administration & dosage* Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / metabolism Cytoskeleton / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / drug effects Drosophila melanogaster / physiology* Drug Resistance Memory / drug effects Memory / physiology* Mushroom Bodies / physiology Signal Transduction rho-Associated Kinases / genetics rho-Associated Kinases / metabolism*
IF 9.412
引用数 4
リソース情報
ショウジョウバエ 9774R-3