RRC ID 46729
著者 Large EE, Mathies LD.
タイトル hunchback and Ikaros-like zinc finger genes control reproductive system development in Caenorhabditis elegans.
ジャーナル Dev Biol
Abstract Here we provide evidence for a C2H2 zinc finger gene family with similarity to Ikaros and hunchback. The founding member of this family is Caenorhabditis elegans ehn-3, which has important and poorly understood functions in somatic gonad development. We examined the expression and function of four additional hunchback/Ikaros-like (HIL) genes in C. elegans reproductive system development. Two genes, ehn-3 and R08E3.4, are expressed in somatic gonadal precursors (SGPs) and have overlapping functions in their development. In ehn-3; R08E3.4 double mutants, we find defects in the generation of distal tip cells, anchor cells, and spermatheca; three of the five tissues derived from the SGPs. We provide in vivo evidence that C. elegans HIL proteins have functionally distinct zinc finger domains, with specificity residing in the N-terminal set of four zinc fingers and a likely protein-protein interaction domain provided by the C-terminal pair of zinc fingers. In addition, we find that a chimeric human Ikaros protein containing the N-terminal zinc fingers of EHN-3 functions in C. elegans. Together, these results lend support to the idea that the C. elegans HIL genes and Ikaros have similar functional domains. We propose that hunchback, Ikaros, and the HIL genes arose from a common ancestor that was present prior to the divergence of protostomes and deuterostomes.
巻・号 339(1)
ページ 51-64
公開日 2010-3-1
DOI 10.1016/j.ydbio.2009.12.013
PII S0012-1606(09)01403-1
PMID 20026024
PMC PMC3721651
MeSH Alleles Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / physiology* Genitalia / embryology* Ikaros Transcription Factor / physiology* RNA Interference Reverse Transcriptase Polymerase Chain Reaction Zinc Fingers*
IF 2.896
引用数 7
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm442 tm1692 tm2127