| RRC ID |
46701
|
| 著者 |
Zhang C, Montgomery TA, Gabel HW, Fischer SE, Phillips CM, Fahlgren N, Sullivan CM, Carrington JC, Ruvkun G.
|
| タイトル |
mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
Argonaute-associated siRNAs and Piwi-associated piRNAs have overlapping roles in silencing mobile genetic elements in animals. In Caenorhabditis elegans, mutator (mut) class genes mediate siRNA-guided repression of transposons as well as exogenous RNAi, but their roles in endogenous RNA silencing pathways are not well-understood. To characterize the endogenous small RNAs dependent on mut class genes, small RNA populations from a null allele of mut-16 as well as a regulatory mut-16(mg461) allele that disables only somatic RNAi were subjected to deep sequencing. Additionally, each of the mut class genes was tested for a requirement in 26G siRNA pathways. The results indicate that mut-16 is an essential factor in multiple endogenous germline and somatic siRNA pathways involving several distinct Argonautes and RNA-dependent RNA polymerases. The results also reveal essential roles for mut-2 and mut-7 in the ERGO-1 class 26G siRNA pathway and less critical roles for mut-8, mut-14, and mut-15. We show that transposons are hypersusceptible to mut-16-dependent silencing and identify a requirement for the siRNA machinery in piRNA biogenesis from Tc1 transposons. We also show that the soma-specific mut-16(mg461) mutant allele is present in multiple C. elegans laboratory strains.
|
| 巻・号 |
108(4)
|
| ページ |
1201-8
|
| 公開日 |
2011-1-25
|
| DOI |
10.1073/pnas.1018695108
|
| PII |
1018695108
|
| PMID |
21245313
|
| PMC |
PMC3029761
|
| MeSH |
Alleles
Animals
Blotting, Northern
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics*
DNA Transposable Elements / genetics
Embryo, Nonmammalian / metabolism
Exoribonucleases / genetics
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
Germ Cells / metabolism
Male
Mutation
RNA, Helminth / classification
RNA, Helminth / genetics
RNA, Helminth / metabolism
RNA, Small Interfering / classification
RNA, Small Interfering / genetics*
RNA, Small Interfering / metabolism
RNA-Binding Proteins / genetics
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics*
Species Specificity
|
| IF |
9.412
|
| 引用数 |
56
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm1661
tm1358 |
