RRC ID 46678
著者 Steimel A, Wong L, Najarro EH, Ackley BD, Garriga G, Hutter H.
タイトル The Flamingo ortholog FMI-1 controls pioneer-dependent navigation of follower axons in C. elegans.
ジャーナル Development
Abstract Development of a functional neuronal network during embryogenesis begins with pioneer axons creating a scaffold along which later-outgrowing axons extend. The molecular mechanism used by these follower axons to navigate along pre-existing axons remains poorly understood. We isolated loss-of-function alleles of fmi-1, which caused strong axon navigation defects of pioneer and follower axons in the ventral nerve cord (VNC) of C. elegans. Notably follower axons, which exclusively depend on pioneer axons for correct navigation, frequently separated from the pioneer. fmi-1 is the sole C. elegans ortholog of Drosophila flamingo and vertebrate Celsr genes, and this phenotype defines a new role for this important molecule in follower axon navigation. FMI-1 has a unique and strikingly conserved structure with cadherin and C-terminal G-protein coupled receptor domains and could mediate cell-cell adhesion and signaling functions. We found that follower axon navigation depended on the extracellular but not on the intracellular domain, suggesting that FMI-1 mediates primarily adhesion between pioneer and follower axons. By contrast, pioneer axon navigation required the intracellular domain, suggesting that FMI-1 acts as receptor transducing a signal in this case. Our findings indicate that FMI-1 is a cell-type dependent axon guidance factor with different domain requirements for its different functions in pioneers and followers.
巻・号 137(21)
ページ 3663-73
公開日 2010-11-1
DOI 10.1242/dev.054320
PII dev.054320
PMID 20876647
PMC PMC2959053
MeSH Animals Animals, Genetically Modified Axons / metabolism Axons / physiology* Body Patterning / genetics Body Patterning / physiology Cadherins / chemistry Cadherins / genetics Cadherins / metabolism Cadherins / physiology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Movement / genetics Cell Movement / physiology* Embryo, Nonmammalian Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Models, Biological Neural Pathways / metabolism Neural Pathways / physiology* Neuroanatomical Tract-Tracing Techniques Protein Structure, Tertiary / genetics Protein Structure, Tertiary / physiology
IF 5.611
引用数 52
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm718 tm1422