RRC ID 46650
著者 Matus DQ, Li XY, Durbin S, Agarwal D, Chi Q, Weiss SJ, Sherwood DR.
タイトル In vivo identification of regulators of cell invasion across basement membranes.
ジャーナル Sci Signal
Abstract Cell invasion through basement membranes during development, immune surveillance, and metastasis remains poorly understood. To gain further insight into this key cellular behavior, we performed an in vivo screen for regulators of cell invasion through basement membranes, using the simple model of Caenorhabditis elegans anchor cell invasion, and identified 99 genes that promote invasion, including the genes encoding the chaperonin complex cct. Notably, most of these genes have not been previously implicated in invasive cell behavior. We characterized members of the cct complex and 11 other gene products, determining the distinct aspects of the invasive cascade that they regulate, including formation of a specialized invasive cell membrane and its ability to breach the basement membrane. RNA interference-mediated knockdown of the human orthologs of cct-5 and lit-1, which had not previously been implicated in cell invasion, reduced the invasiveness of metastatic carcinoma cells, suggesting that a conserved genetic program underlies cell invasion. These results increase our understanding of the genetic underpinnings of cell invasion and also provide new potential therapeutic targets to limit this behavior.
巻・号 3(120)
ページ ra35
公開日 2010-5-4
DOI 10.1126/scisignal.2000654
PII 3/120/ra35
PMID 20442418
PMC PMC3917318
MeSH Animals Basement Membrane / cytology* Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Neoplasm Invasiveness* / genetics RNA Interference
IF 6.467
引用数 39
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm3042 tm3126 tm3082