論文 - 詳細
| RRC ID | 46647 |
|---|---|
| 著者 | Maniar JM, Fire AZ. |
| タイトル | EGO-1, a C. elegans RdRP, modulates gene expression via production of mRNA-templated short antisense RNAs. |
| ジャーナル | Curr Biol |
| Abstract |
BACKGROUND:The development of the germline in Caenorhabditis elegans is a complex process involving the regulation of thousands of genes in a coordinated manner. Several genes required for small RNA biogenesis and function are among those required for the proper organization of the germline. EGO-1 is a putative RNA-directed RNA polymerase (RdRP) that is required for multiple aspects of C. elegans germline development and efficient RNA interference (RNAi) of germline-expressed genes. RdRPs have been proposed to act through a variety of mechanisms, including the posttranscriptional targeting of specific mRNAs, as well as through a direct interaction with chromatin. Despite extensive investigation, the molecular role of EGO-1 has remained enigmatic. RESULTS:Here we use high-throughput small RNA and messenger RNA sequencing to investigate EGO-1 function. We found that EGO-1 is required to produce a distinct pool of small RNAs antisense to a number of germline-expressed mRNAs through several developmental stages. These potential mRNA targets fall into distinct classes, including genes required for kinetochore and nuclear pore assembly, histone-modifying activities, and centromeric proteins. We also found several RNAi-related genes to be targets of EGO-1. Finally, we show a strong association between the loss of small RNAs and the rise of mRNA levels in ego-1(-) animals. CONCLUSIONS:Our data support the conclusion that EGO-1 produces triphosphorylated small RNAs derived from mRNA templates and that these small RNAs modulate gene expression through the targeting of their cognate mRNAs. |
| 巻・号 | 21(6) |
| ページ | 449-59 |
| 公開日 | 2011-3-22 |
| DOI | 10.1016/j.cub.2011.02.019 |
| PII | S0960-9822(11)00210-7 |
| PMID | 21396820 |
| PMC | PMC3073447 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation / physiology* RNA Interference* RNA, Messenger / analysis* RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics RNA-Dependent RNA Polymerase / metabolism* Sequence Analysis, RNA |
| IF | 9.601 |
| 引用数 | 44 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm892 tm1599 |