RRC ID 46588
著者 Fleming T, Chien SC, Vanderzalm PJ, Dell M, Gavin MK, Forrester WC, Garriga G.
タイトル The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility.
ジャーナル Dev Biol
Abstract Ena/VASP proteins mediate the effects of guidance cues on the actin cytoskeleton. The single C. elegans homolog of the Ena/VASP family of proteins, UNC-34, is required for the migrations of cells and growth cones. Here we show that unc-34 mutant alleles also interact genetically with Wnt mutants to reveal a role for unc-34 in the establishment of neuronal polarity along the C. elegans anterior-posterior axis. Our mutant analysis shows that eliminating UNC-34 function results in neuronal migration and polarity phenotypes that are enhanced at higher temperatures, revealing a heat-sensitive process that is normally masked by the presence of UNC-34. Finally, we show that the UNC-34 protein is expressed broadly and accumulates in axons and at the apical junctions of epithelial cells. While most mutants lacked detectable UNC-34, three unc-34 mutants that contained missense mutations in the EVH1 domain produced full-length UNC-34 that failed to localize to apical junctions and axons, supporting the role for the EVH1 domain in localizing Ena/VASP family members.
巻・号 344(1)
ページ 94-106
公開日 2010-8-1
DOI 10.1016/j.ydbio.2010.04.025
PII S0012-1606(10)00270-8
PMID 20452341
PMC PMC2909364
MeSH Alleles Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism* Cell Movement Cloning, Molecular Cytoskeleton / metabolism Gene Expression Regulation, Developmental* Hot Temperature Models, Biological Mutation Nerve Tissue Proteins / metabolism* Neurons / metabolism* Phenotype Protein Structure, Tertiary RNA Interference
IF 2.896
引用数 18
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm597