RRC ID |
46588
|
著者 |
Fleming T, Chien SC, Vanderzalm PJ, Dell M, Gavin MK, Forrester WC, Garriga G.
|
タイトル |
The role of C. elegans Ena/VASP homolog UNC-34 in neuronal polarity and motility.
|
ジャーナル |
Dev Biol
|
Abstract |
Ena/VASP proteins mediate the effects of guidance cues on the actin cytoskeleton. The single C. elegans homolog of the Ena/VASP family of proteins, UNC-34, is required for the migrations of cells and growth cones. Here we show that unc-34 mutant alleles also interact genetically with Wnt mutants to reveal a role for unc-34 in the establishment of neuronal polarity along the C. elegans anterior-posterior axis. Our mutant analysis shows that eliminating UNC-34 function results in neuronal migration and polarity phenotypes that are enhanced at higher temperatures, revealing a heat-sensitive process that is normally masked by the presence of UNC-34. Finally, we show that the UNC-34 protein is expressed broadly and accumulates in axons and at the apical junctions of epithelial cells. While most mutants lacked detectable UNC-34, three unc-34 mutants that contained missense mutations in the EVH1 domain produced full-length UNC-34 that failed to localize to apical junctions and axons, supporting the role for the EVH1 domain in localizing Ena/VASP family members.
|
巻・号 |
344(1)
|
ページ |
94-106
|
公開日 |
2010-8-1
|
DOI |
10.1016/j.ydbio.2010.04.025
|
PII |
S0012-1606(10)00270-8
|
PMID |
20452341
|
PMC |
PMC2909364
|
MeSH |
Alleles
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins / metabolism*
Cell Movement
Cloning, Molecular
Cytoskeleton / metabolism
Gene Expression Regulation, Developmental*
Hot Temperature
Models, Biological
Mutation
Nerve Tissue Proteins / metabolism*
Neurons / metabolism*
Phenotype
Protein Structure, Tertiary
RNA Interference
|
IF |
2.896
|
引用数 |
18
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
線虫 |
tm597 |