RRC ID 46570
著者 Berdichevsky A, Nedelcu S, Boulias K, Bishop NA, Guarente L, Horvitz HR.
タイトル 3-Ketoacyl thiolase delays aging of Caenorhabditis elegans and is required for lifespan extension mediated by sir-2.1.
ジャーナル Proc Natl Acad Sci U S A
Abstract Studies of long-lived Caenorhabditis elegans mutants have identified several genes that function to limit lifespan, i.e., loss-of-function mutations in these genes promote longevity. By contrast, little is known about genes that normally act to delay aging and that when mutated cause premature aging (progeria). To seek such genes, we performed a genetic screen for C. elegans mutants that age prematurely. We found that loss-of-function mutations of the ketoacyl thiolase gene kat-1 result in an increased accumulation of the lipofuscin-like fluorescent aging pigment, shortened lifespan, early behavioral decline, and other abnormalities characteristic of premature aging. These findings suggest that kat-1 acts to delay C. elegans aging. kat-1 encodes a conserved metabolic enzyme that catalyzes the last step of fatty acid oxidation and was previously shown to regulate fat accumulation in worms. We observed that kat-1 is required for the extension of lifespan and enhanced thermotolerance mediated by extra copies of the deacetylase gene sir-2.1. kat-1 acts independently of other known pathways that affect longevity. Our findings suggest that defects in fatty acid oxidation can limit lifespan and accelerate aging in C. elegans and that kat-1-mediated fatty acid oxidation is crucial for overexpressed sir-2.1 to delay aging.
巻・号 107(44)
ページ 18927-32
公開日 2010-11-2
DOI 10.1073/pnas.1013854107
PII 1013854107
PMID 20956318
PMC PMC2973869
MeSH Acetyl-CoA C-Acyltransferase / genetics Acetyl-CoA C-Acyltransferase / metabolism* Aging / physiology* Aging, Premature / enzymology Aging, Premature / genetics Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Fatty Acids / genetics Fatty Acids / metabolism* Lipofuscin / genetics Lipofuscin / metabolism Mutation Oxidation-Reduction Progeria / enzymology Progeria / genetics Sirtuins / genetics Sirtuins / metabolism*
IF 9.412
引用数 25
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1037