RRC ID |
46565
|
著者 |
Asikainen S, Rudgalvyte M, Heikkinen L, Louhiranta K, Lakso M, Wong G, Nass R.
|
タイトル |
Global microRNA expression profiling of Caenorhabditis elegans Parkinson's disease models.
|
ジャーナル |
J Mol Neurosci
|
Abstract |
MicroRNAs (miRNAs) play an important role in human brain development and maintenance. To search for miRNAs that may be involved in the pathogenesis of Parkinsons disease (PD), we utilized miRNA microarrays to identify potential gene expression changes in 115 annotated miRNAs in PD-associated Caenorhabditis elegans models that either overexpress human A53T alpha-synuclein or have mutations within the vesicular catecholamine transporter (cat-1) or parkin (pdr-1) ortholog. Here, we show that 12 specific miRNAs are differentially regulated in the animals overexpressing alpha-synuclein, five in cat-1, and three in the pdr-1 mutants. The family of miR-64 and miR-65 are co-underexpressed in the alpha-synuclein transgenic and cat-1 strains, and members of let-7 family co-underexpressed in the alpha-synuclein and pdr-1 strains; mdl-1 and ptc-1 genes are target candidates for miR-64 and miR-65 and are overexpressed in alpha-synuclein transgenic as well as miR-64/65 (tm3711) knockout animals. These results indicate that miRNAs are differentially expressed in C. elegans PD models and suggest a role for these molecules in disease pathogenesis.
|
巻・号 |
41(1)
|
ページ |
210-8
|
公開日 |
2010-5-1
|
DOI |
10.1007/s12031-009-9325-1
|
PMID |
20091141
|
MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Disease Models, Animal*
Gene Expression Profiling
Humans
MicroRNAs* / genetics
MicroRNAs* / metabolism
Oligonucleotide Array Sequence Analysis / methods
Parkinson Disease / genetics*
Parkinson Disease / pathology
Parkinson Disease / physiopathology
|
IF |
2.678
|
引用数 |
70
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
NEUROSCIENCES
|
リソース情報 |
線虫 |
tm3711 |