| RRC ID |
46540
|
| 著者 |
Yee CS, Sybingco SS, Serdetchania V, Kholkina G, Bueno de Mesquita M, Naqvi Z, Park SH, Lam K, Killeen MT.
|
| タイトル |
ENU-3 is a novel motor axon outgrowth and guidance protein in C. elegans.
|
| ジャーナル |
Dev Biol
|
| Abstract |
During the development of the nervous system, the migration of many cells and axons is guided by extracellular molecules. These molecules bind to receptors at the tips of the growth cones of migrating axons and trigger intracellular signaling to steer the axons along the correct trajectories. We have identified a novel mutant, enu-3 (enhancer of Unc), that enhances the motor neuron axon outgrowth defects observed in strains of Caenorhabditis elegans that lack either the UNC-5 receptor or its ligand UNC-6/Netrin. Specifically, the double-mutant strains have enhanced axonal outgrowth defects mainly in DB4, DB5 and DB6 motor neurons. enu-3 single mutants have weak motor neuron axon migration defects. Both outgrowth defects of double mutants and axon migration defects of enu-3 mutants were rescued by expression of the H04D03.1 gene product. ENU-3/H04D03.1 encodes a novel predicted putative trans-membrane protein of 204 amino acids. It is a member of a family of highly homologous proteins of previously unknown function in the C. elegans genome. ENU-3 is expressed in the PVT interneuron and is weakly expressed in many cell bodies along the ventral cord, including those of the DA and DB motor neurons. We conclude that ENU-3 is a novel C. elegans protein that affects both motor axon outgrowth and guidance.
|
| 巻・号 |
352(2)
|
| ページ |
243-53
|
| 公開日 |
2011-4-15
|
| DOI |
10.1016/j.ydbio.2011.01.024
|
| PII |
S0012-1606(11)00056-X
|
| PMID |
21295567
|
| MeSH |
Amino Acid Sequence
Animals
Axons / physiology
Base Sequence
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / chemistry
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / physiology*
Chromosome Mapping
DNA Primers / genetics
Gene Expression Regulation, Developmental
Membrane Proteins / chemistry
Membrane Proteins / genetics*
Membrane Proteins / physiology*
Molecular Sequence Data
Motor Neurons / physiology
Mutation
Nerve Tissue Proteins / chemistry
Nerve Tissue Proteins / genetics*
Nerve Tissue Proteins / physiology*
Neurogenesis / genetics
Neurogenesis / physiology
Sequence Homology, Amino Acid
|
| IF |
2.896
|
| 引用数 |
6
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm4519 |
