| RRC ID |
46472
|
| 著者 |
Xie L, Gao S, Alcaire SM, Aoyagi K, Wang Y, Griffin JK, Stagljar I, Nagamatsu S, Zhen M.
|
| タイトル |
NLF-1 delivers a sodium leak channel to regulate neuronal excitability and modulate rhythmic locomotion.
|
| ジャーナル |
Neuron
|
| Abstract |
A cation channel NCA/UNC-79/UNC-80 affects neuronal activity. We report here the identification of a conserved endoplasmic reticulum protein NLF-1 (NCA localization factor-1) that regulates neuronal excitability and locomotion through the NCA channel. In C. elegans, the loss of either NLF-1 or NCA leads to a reduced sodium leak current, and a hyperpolarized resting membrane potential in premotor interneurons. This results in a decreased premotor interneuron activity that reduces the initiation and sustainability of rhythmic locomotion. NLF-1 promotes axonal localization of all NCA reporters. Its mouse homolog mNLF-1 functionally substitutes for NLF-1 in C. elegans, interacts with the mammalian sodium leak channel NALCN in vitro, and potentiates sodium leak currents in primary cortical neuron cultures. Taken together, an ER protein NLF-1 delivers a sodium leak channel to maintain neuronal excitability and potentiates a premotor interneuron network critical for C. elegans rhythmic locomotion.
|
| 巻・号 |
77(6)
|
| ページ |
1069-82
|
| 公開日 |
2013-3-20
|
| DOI |
10.1016/j.neuron.2013.01.018
|
| PII |
S0896-6273(13)00084-6
|
| PMID |
23522043
|
| MeSH |
Animals
Axons / metabolism
Caenorhabditis elegans
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Caenorhabditis elegans Proteins / physiology
Cells, Cultured
Endoplasmic Reticulum / metabolism
Gene Knockdown Techniques / methods
Ion Channels
Locomotion / physiology*
Membrane Proteins
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Neurons / metabolism*
Nuclear Proteins
Periodicity*
Sodium Channels / genetics
Sodium Channels / metabolism*
Sodium Channels / physiology
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcription Factors / physiology
|
| IF |
14.415
|
| 引用数 |
35
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| 線虫 |
tm3631 |
