RRC ID 46464
著者 Warf MB, Shepherd BA, Johnson WE, Bass BL.
タイトル Effects of ADARs on small RNA processing pathways in C. elegans.
ジャーナル Genome Res
Abstract Adenosine deaminases that act on RNA (ADARs) are RNA editing enzymes that convert adenosine to inosine in double-stranded RNA (dsRNA). To evaluate effects of ADARs on small RNAs that derive from dsRNA precursors, we performed deep-sequencing, comparing small RNAs from wild-type and ADAR mutant Caenorhabditis elegans. While editing in small RNAs was rare, at least 40% of microRNAs had altered levels in at least one ADAR mutant strain, and miRNAs with significantly altered levels had mRNA targets with correspondingly affected levels. About 40% of siRNAs derived from endogenous genes (endo-siRNAs) also had altered levels in at least one mutant strain, including 63% of Dicer-dependent endo-siRNAs. The 26G class of endo-siRNAs was significantly affected by ADARs, and many altered 26G loci had intronic reads and histone modifications associated with transcriptional silencing. Our data indicate that ADARs, through both direct and indirect mechanisms, are important for maintaining wild-type levels of many small RNAs in C. elegans.
巻・号 22(8)
ページ 1488-98
公開日 2012-8-1
DOI 10.1101/gr.134841.111
PII gr.134841.111
PMID 22673872
PMC PMC3409262
MeSH Adenosine Deaminase / genetics Adenosine Deaminase / metabolism* Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Gene Expression Regulation, Enzymologic* Gene Silencing Genetic Loci High-Throughput Nucleotide Sequencing / methods Histones / genetics Histones / metabolism MicroRNAs / genetics MicroRNAs / metabolism* RNA Processing, Post-Transcriptional* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism RNA-Binding Proteins Ribonuclease III / genetics Ribonuclease III / metabolism
IF 11.093
引用数 34
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm668