論文 - 詳細
| RRC ID | 46432 |
|---|---|
| 著者 | Sann SB, Crane MM, Lu H, Jin Y. |
| タイトル | Rabx-5 regulates RAB-5 early endosomal compartments and synaptic vesicles in C. elegans. |
| ジャーナル | PLoS One |
| Abstract |
Early endosomal membrane compartments are required for the formation and recycling of synaptic vesicles, but how these compartments are regulated is incompletely understood. We performed a forward genetic screen in C. elegans for mutations that affect RAB-5 labeled early endosomal compartments in GABAergic motoneurons. Here we report the isolation and characterization of one mutation, rabx-5. The rabx-5 mutation leads to decreased intensity of YFP::RAB-5 in the cell soma but increased intensity in the synaptic and intersynaptic regions of the axon. This effect is due to the bias of the cycling state of RAB-5, and results from a change in the organization of the early endosomal compartment as well as the membrane binding state of RAB-5. Synaptic vesicle accumulation is altered in rabx-5 mutants, and synaptic transmission from cholinergic neurons is decreased. Early endosomal membrane compartments show disorganization with ageing and rabx-5 mutant animals age faster. These results suggest that rabx-5 regulation of RAB-5 compartments is important for maintaining proper synaptic function throughout the lifetime. |
| 巻・号 | 7(6) |
| ページ | e37930 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0037930 |
| PII | PONE-D-12-07814 |
| PMID | 22675499 |
| PMC | PMC3366993 |
| MeSH | Aging / metabolism Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Compartmentation* Endocytosis Endosomes / metabolism* GABAergic Neurons / cytology GABAergic Neurons / metabolism Genes, Helminth / genetics Guanine Nucleotide Exchange Factors / chemistry Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism* Motor Neurons / cytology Motor Neurons / metabolism Mutation / genetics Phenotype Protein Binding Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins / metabolism Synaptic Vesicles / metabolism* Vesicular Transport Proteins / metabolism* |
| IF | 2.74 |
| 引用数 | 11 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm1512 tm1555 |