RRC ID 46417
著者 Nishio N, Mohri-Shiomi A, Nishida Y, Hiramatsu N, Kodama-Namba E, Kimura KD, Kuhara A, Mori I.
タイトル A novel and conserved protein AHO-3 is required for thermotactic plasticity associated with feeding states in Caenorhabditis elegans.
ジャーナル Genes Cells
Abstract Although a large proportion of molecules expressed in the nervous system are conserved from invertebrate to vertebrate, functional properties of such molecules are less characterized. Here, we show that highly conserved hydrolase AHO-3 acts as a novel regulator of starvation-induced thermotactic plasticity in Caenorhabditis elegans. As wild-type animals, aho-3 mutants migrated to the cultivation temperature on a linear thermal gradient after cultivation at a particular temperature with food. Whereas wild-type animals cultivated under food-deprived condition showed dispersed distribution on the gradient, aho-3 mutants exhibited tendency to migrate toward higher temperature. Such an abnormal behavior was completely rescued by the expression of human homologue of AHO-3, indicating that the molecular function of AHO-3 is highly conserved between nematode and human. The behavioral regulation by AHO-3 requires the N-terminal cysteine cluster, which ensures the proper subcellular localization of AHO-3 to sensory endings. Double-mutant analysis suggested that AHO-3 acts in the same pathway with ODR-3, a heterotrimeric G protein alpha subunit. Our results unveiled a novel neural protein in C. elegans, confirming its conserved role in behavioral regulation.
巻・号 17(5)
ページ 365-86
公開日 2012-5-1
DOI 10.1111/j.1365-2443.2012.01594.x
PMID 22512337
PMC PMC3506735
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Behavior, Animal / physiology Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Food Deprivation Humans Hydrolases / genetics* Hydrolases / metabolism Locomotion / physiology Molecular Sequence Data Sequence Alignment Temperature
IF 1.655
引用数 6
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
線虫 tm2411 tm501