RRC ID 46409
著者 Mikeladze-Dvali T, von Tobel L, Strnad P, Knott G, Leonhardt H, Schermelleh L, Gönczy P.
タイトル Analysis of centriole elimination during C. elegans oogenesis.
ジャーナル Development
Abstract Centrosomes are the principal microtubule organizing centers (MTOCs) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated, notably to ensure bipolar spindle formation and thus faithful chromosome segregation. In the germ line of most metazoan species, centrioles are maintained during spermatogenesis, but eliminated during oogenesis. Such differential behavior ensures that the appropriate number of centrioles is present in the newly fertilized zygote. Despite being a fundamental feature of sexual reproduction in metazoans, the mechanisms governing centriole elimination during oogenesis are poorly understood. Here, we investigate this question in C. elegans. Using antibodies directed against centriolar components and serial-section electron microscopy, we establish that centrioles are eliminated during the diplotene stage of the meiotic cell cycle. Moreover, we show that centriole elimination is delayed upon depletion of the helicase CGH-1. We also find that somatic cells make a minor contribution to this process, and demonstrate that the germ cell karyotype is important for timely centriole elimination. These findings set the stage for a mechanistic dissection of centriole elimination in a metazoan organism.
巻・号 139(9)
ページ 1670-9
公開日 2012-5-1
DOI 10.1242/dev.075440
PII 139/9/1670
PMID 22492357
PMC PMC4074223
MeSH Animals Caenorhabditis elegans / physiology* Centrioles / physiology* Centrioles / ultrastructure Female Fluorescence Recovery After Photobleaching Fluorescent Antibody Technique, Indirect Karyotyping Meiotic Prophase I / physiology* Microscopy, Electron, Transmission Oogenesis / physiology* RNA Interference
IF 5.611
引用数 43
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm3951 tm1753