RRC ID 46335
著者 Sanchez-Alvarez L, Visanuvimol J, McEwan A, Su A, Imai JH, Colavita A.
タイトル VANG-1 and PRKL-1 cooperate to negatively regulate neurite formation in Caenorhabditis elegans.
ジャーナル PLoS Genet
Abstract Neuritogenesis is a critical early step in the development and maturation of neurons and neuronal circuits. While extracellular directional cues are known to specify the site and orientation of nascent neurite formation in vivo, little is known about the genetic pathways that block inappropriate neurite emergence in order to maintain proper neuronal polarity. Here we report that the Caenorhabditis elegans orthologues of Van Gogh (vang-1), Prickle (prkl-1), and Dishevelled (dsh-1), core components of planar cell polarity (PCP) signaling, are required in a subset of peripheral motor neurons to restrict neurite emergence to a specific organ axis. In loss-of-function mutants, neurons display supernumerary neurites that extend inappropriately along the orthogonal anteroposterior (A/P) body axis. We show that autonomous and non-autonomous gene activities are required early and persistently to inhibit the formation or consolidation of growth cone protrusions directed away from organ precursor cells. Furthermore, prkl-1 overexpression is sufficient to suppress neurite formation and reorient neuronal polarity in a vang-1- and dsh-1-dependent manner. Our findings suggest a novel role for a PCP-like pathway in maintaining polarized neuronal morphology by inhibiting neuronal responses to extrinsic or intrinsic cues that would otherwise promote extraneous neurite formation.
巻・号 7(9)
ページ e1002257
公開日 2011-9-1
DOI 10.1371/journal.pgen.1002257
PII PGENETICS-D-11-00449
PMID 21912529
PMC PMC3164692
MeSH Animals Body Patterning / genetics* Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Polarity / genetics* Dishevelled Proteins Gene Expression Regulation, Developmental Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Neurites / physiology* Neurogenesis / genetics Neurons / cytology Neurons / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism* RNA Interference Signal Transduction
IF 5.175
引用数 21
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm1422 tm2639 tm306