RRC ID 46333
著者 Rutkowski R, Dickinson R, Stewart G, Craig A, Schimpl M, Keyse SM, Gartner A.
タイトル Regulation of Caenorhabditis elegans p53/CEP-1-dependent germ cell apoptosis by Ras/MAPK signaling.
ジャーナル PLoS Genet
Abstract Maintaining genome stability in the germline is thought to be an evolutionarily ancient role of the p53 family. The sole Caenorhabditis elegans p53 family member CEP-1 is required for apoptosis induction in meiotic, late-stage pachytene germ cells in response to DNA damage and meiotic recombination failure. In an unbiased genetic screen for negative regulators of CEP-1, we found that increased activation of the C. elegans ERK orthologue MPK-1, resulting from either loss of the lip-1 phosphatase or activation of let-60 Ras, results in enhanced cep-1-dependent DNA damage induced apoptosis. We further show that MPK-1 is required for DNA damage-induced germ cell apoptosis. We provide evidence that MPK-1 signaling regulates the apoptotic competency of germ cells by restricting CEP-1 protein expression to cells in late pachytene. Restricting CEP-1 expression to cells in late pachytene is thought to ensure that apoptosis doesn't occur in earlier-stage cells where meiotic recombination occurs. MPK-1 signaling regulates CEP-1 expression in part by regulating the levels of GLD-1, a translational repressor of CEP-1, but also via a GLD-1-independent mechanism. In addition, we show that MPK-1 is phosphorylated and activated upon ionising radiation (IR) in late pachytene germ cells and that MPK-1-dependent CEP-1 activation may be in part direct, as these two proteins interact in a yeast two-hybrid assay. In summary, we report our novel finding that MAP kinase signaling controls CEP-1-dependent apoptosis by several different pathways that converge on CEP-1. Since apoptosis is also restricted to pachytene stage cells in mammalian germlines, analogous mechanisms regulating p53 family members are likely to be conserved throughout evolution.
巻・号 7(8)
ページ e1002238
公開日 2011-8-1
DOI 10.1371/journal.pgen.1002238
PII PGENETICS-D-11-00390
PMID 21901106
PMC PMC3161941
MeSH Animals Apoptosis* Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* DNA Damage Gene Expression Regulation, Developmental Genes, p53 Germ Cells / cytology Germ Cells / metabolism MAP Kinase Signaling System Meiosis Mitogen-Activated Protein Kinase 1 / genetics Mitogen-Activated Protein Kinase 1 / metabolism* Pachytene Stage / genetics Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism* Signal Transduction Tumor Suppressor Protein p53 / genetics Tumor Suppressor Protein p53 / metabolism* Two-Hybrid System Techniques ras Proteins / genetics ras Proteins / metabolism*
IF 5.175
引用数 43
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm853