| RRC ID |
46304
|
| 著者 |
Cox-Paulson EA, Walck-Shannon E, Lynch AM, Yamashiro S, Zaidel-Bar R, Eno CC, Ono S, Hardin J.
|
| タイトル |
Tropomodulin protects α-catenin-dependent junctional-actin networks under stress during epithelial morphogenesis.
|
| ジャーナル |
Curr Biol
|
| Abstract |
α-catenin is central to recruitment of actin networks to the cadherin-catenin complex, but how such networks are subsequently stabilized against stress applied during morphogenesis is poorly understood. To identify proteins that functionally interact with α-catenin in this process, we performed enhancer screening using a weak allele of the C. elegans α-catenin, hmp-1, thereby identifying UNC-94/tropomodulin. Tropomodulins (Tmods) cap the minus ends of F-actin in sarcomeres. They also regulate lamellipodia, can promote actin nucleation, and are required for normal cardiovascular development and neuronal growth-cone morphology. Tmods regulate the morphology of cultured epithelial cells, but their role in epithelia in vivo remains unexplored. We find that UNC-94 is enriched within a HMP-1-dependent junctional-actin network at epidermal adherens junctions subject to stress during morphogenesis. Loss of UNC-94 leads to discontinuity of this network, and high-speed filming of hmp-1(fe4);unc-94(RNAi) embryos reveals large junctional displacements that depend on the Rho pathway. In vitro, UNC-94 acts in combination with HMP-1, leading to longer actin bundles than with HMP-1 alone. Our data suggest that Tmods protect actin filaments recruited by α-catenin from minus-end subunit loss, enabling them to withstand the stresses of morphogenesis.
|
| 巻・号 |
22(16)
|
| ページ |
1500-5
|
| 公開日 |
2012-8-21
|
| DOI |
10.1016/j.cub.2012.06.025
|
| PII |
S0960-9822(12)00670-7
|
| PMID |
22771044
|
| PMC |
PMC3427464
|
| MeSH |
Actins / metabolism*
Animals
Caenorhabditis elegans
Epidermis / embryology
Morphogenesis*
Stress, Mechanical*
Tropomodulin / metabolism*
alpha Catenin / metabolism*
|
| IF |
9.601
|
| 引用数 |
19
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm724 |
