論文 - 詳細
| RRC ID | 46276 |
|---|---|
| 著者 | Burger J, Merlet J, Tavernier N, Richaudeau B, Arnold A, Ciosk R, Bowerman B, Pintard L. |
| タイトル | CRL2(LRR-1) E3-ligase regulates proliferation and progression through meiosis in the Caenorhabditis elegans germline. |
| ジャーナル | PLoS Genet |
| Abstract |
The ubiquitin-proteolytic system controls the stability of proteins in space and time. In this study, using a temperature-sensitive mutant allele of the cul-2 gene, we show that CRL2(LRR-1) (CUL-2 RING E3 ubiquitin-ligase and the Leucine Rich Repeat 1 substrate recognition subunit) acts at multiple levels to control germline development. CRL2(LRR-1) promotes germ cell proliferation by counteracting the DNA replication ATL-1 checkpoint pathway. CRL2(LRR-1) also participates in the mitotic proliferation/meiotic entry decision, presumably controlling the stability of meiotic promoting factors in the mitotic zone of the germline. Finally, CRL2(LRR-1) inhibits the first steps of meiotic prophase by targeting in mitotic germ cells degradation of the HORMA domain-containing protein HTP-3, required for loading synaptonemal complex components onto meiotic chromosomes. Given its widespread evolutionary conservation, CUL-2 may similarly regulate germline development in other organisms as well. |
| 巻・号 | 9(3) |
| ページ | e1003375 |
| 公開日 | 2013-3-1 |
| DOI | 10.1371/journal.pgen.1003375 |
| PII | PGENETICS-D-12-01810 |
| PMID | 23555289 |
| PMC | PMC3610609 |
| MeSH | Animals Ataxia Telangiectasia Mutated Proteins Caenorhabditis elegans* / cytology Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Cycle / genetics Cell Cycle Proteins / metabolism Cell Proliferation* Cullin Proteins* / genetics Cullin Proteins* / metabolism DNA Replication Germ Cells / cytology Germ Cells / metabolism Meiosis / genetics* Mitosis Phosphotransferases / metabolism Synaptonemal Complex / metabolism |
| IF | 5.175 |
| 引用数 | 23 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 線虫 | tm3543 |