論文 - 詳細
RRC ID | 46247 |
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著者 | Zhang P, Zhang H. |
タイトル | Autophagy modulates miRNA-mediated gene silencing and selectively degrades AIN-1/GW182 in C. elegans. |
ジャーナル | EMBO Rep |
Abstract |
MicroRNAs (miRNAs) post-transcriptionally repress gene expression via the miRNA-induced silencing complex (miRISC), which includes miRNA, Argonaute and a GW182 family member. Here we show that in Caenorhabditis elegans, miRNA-mediated gene silencing is modulated by macroautophagy, a lysosome-mediated degradation process. Loss of autophagy activity suppresses developmental defects caused by partially impaired silencing of miRNA targets including the let-7 family and lsy-6. The C. elegans GW182 homolog AIN-1 is itself selectively degraded by autophagy and colocalizes with the p62 homolog SQST-1 in autophagy mutants. Thus, autophagy activity modulates miRNA-mediated gene silencing and degrades a core miRISC component. |
巻・号 | 14(6) |
ページ | 568-76 |
公開日 | 2013-6-1 |
DOI | 10.1038/embor.2013.53 |
PII | embor201353 |
PMID | 23619095 |
PMC | PMC3674441 |
MeSH | Animals Autophagy* Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Carrier Proteins / genetics* Carrier Proteins / metabolism Gene Expression Regulation, Developmental Larva / cytology Larva / genetics Larva / growth & development MicroRNAs / genetics* Mutation Proteolysis RNA Interference* RNA-Induced Silencing Complex / genetics ras Proteins / genetics ras Proteins / metabolism |
IF | 7.497 |
引用数 | 35 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
線虫 | tm3425 tm2508 |