| RRC ID |
46245
|
| 著者 |
Yu H, Aleman-Meza B, Gharib S, Labocha MK, Cronin CJ, Sternberg PW, Zhong W.
|
| タイトル |
Systematic profiling of Caenorhabditis elegans locomotive behaviors reveals additional components in G-protein Gαq signaling.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
Genetic screens have been widely applied to uncover genetic mechanisms of movement disorders. However, most screens rely on human observations of qualitative differences. Here we demonstrate the application of an automatic imaging system to conduct a quantitative screen for genes regulating the locomotive behavior in Caenorhabditis elegans. Two hundred twenty-seven neuronal signaling genes with viable homozygous mutants were selected for this study. We tracked and recorded each animal for 4 min and analyzed over 4,400 animals of 239 genotypes to obtain a quantitative, 10-parameter behavioral profile for each genotype. We discovered 87 genes whose inactivation causes movement defects, including 50 genes that had never been associated with locomotive defects. Computational analysis of the high-content behavioral profiles predicted 370 genetic interactions among these genes. Network partition revealed several functional modules regulating locomotive behaviors, including sensory genes that detect environmental conditions, genes that function in multiple types of excitable cells, and genes in the signaling pathway of the G protein Gαq, a protein that is essential for animal life and behavior. We developed quantitative epistasis analysis methods to analyze the locomotive profiles and validated the prediction of the γ isoform of phospholipase C as a component in the Gαq pathway. These results provided a system-level understanding of how neuronal signaling genes coordinate locomotive behaviors. This study also demonstrated the power of quantitative approaches in genetic studies.
|
| 巻・号 |
110(29)
|
| ページ |
11940-5
|
| 公開日 |
2013-7-16
|
| DOI |
10.1073/pnas.1310468110
|
| PII |
1310468110
|
| PMID |
23818641
|
| PMC |
PMC3718121
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / physiology*
Epistasis, Genetic / genetics
Epistasis, Genetic / physiology*
GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism*
Gene Expression Profiling / methods
Locomotion / genetics
Locomotion / physiology*
Neurons / metabolism*
RNA Interference
Signal Transduction / genetics
Signal Transduction / physiology*
|
| IF |
9.412
|
| 引用数 |
16
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 線虫 |
tm1340
tm863
tm1392
tm1108
tm1133
tm2416
tm1661
tm698
tm805
tm1325 |
