RRC ID 46172
著者 Richardson CE, Spilker KA, Cueva JG, Perrino J, Goodman MB, Shen K.
タイトル PTRN-1, a microtubule minus end-binding CAMSAP homolog, promotes microtubule function in Caenorhabditis elegans neurons.
ジャーナル Elife
Abstract In neuronal processes, microtubules (MTs) provide structural support and serve as tracks for molecular motors. While it is known that neuronal MTs are more stable than MTs in non-neuronal cells, the molecular mechanisms underlying this stability are not fully understood. In this study, we used live fluorescence microscopy to show that the C. elegans CAMSAP protein PTRN-1 localizes to puncta along neuronal processes, stabilizes MT foci, and promotes MT polymerization in neurites. Electron microscopy revealed that ptrn-1 null mutants have fewer MTs and abnormal MT organization in the PLM neuron. Animals grown with a MT depolymerizing drug caused synthetic defects in neurite branching in the absence of ptrn-1 function, indicating that PTRN-1 promotes MT stability. Further, ptrn-1 null mutants exhibited aberrant neurite morphology and synaptic vesicle localization that is partially dependent on dlk-1. Our results suggest that PTRN-1 represents an important mechanism for promoting MT stability in neurons. DOI: http://dx.doi.org/10.7554/eLife.01498.001.
巻・号 3
ページ e01498
公開日 2014-2-25
DOI 10.7554/eLife.01498
PMID 24569477
PMC PMC3932522
MeSH Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Genotype MAP Kinase Kinase Kinases / metabolism Microscopy, Electron Microscopy, Fluorescence Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / drug effects Microtubules / metabolism* Mutation Neurites / metabolism Neurons / drug effects Neurons / metabolism* Phenotype Signal Transduction Synaptic Vesicles / metabolism Tubulin Modulators / pharmacology
IF 7.08
引用数 51
WOS 分野 BIOLOGY
リソース情報
線虫 tm5597