論文 - 詳細
| RRC ID | 46153 |
|---|---|
| 著者 | Li YX, Wang Y, Hu YO, Zhong JX, Wang DY. |
| タイトル | Modulation of the assay system for the sensory integration of 2 sensory stimuli that inhibit each other in nematode Caenorhabditis elegans. |
| ジャーナル | Neurosci Bull |
| Abstract |
OBJECTIVE:To perform the modulation of an assay system for the sensory integration of 2 sensory stimuli that inhibit each other. METHODS:The assay system for assessing the integrative response to 2 reciprocally-inhibitory sensory stimuli was modulated by changing the metal ion barrier. Moreover, the hen-1, ttx-3 and casy-1 mutants having known defects in integrative response were used to evaluate the modulated assay systems. Based on the examined assay systems, new genes possibly involved in the sensory integration control were identified. RESULTS:In the presence of different metal ion barriers and diacetyl, locomotion behaviors, basic movements, pan-neuronal, cholinergic and GABAergic neuronal GFP expressions, neuronal development, structures of sensory neurons and interneurons, and stress response of nematodes in different regions of examined assay systems were normal, and chemotaxis toward different concentrations of diacetyl and avoidance of different concentrations of metal ions were inhibited. In the first group, most of the nematodes moved to diacetyl by crossing the barrier of Fe(2+), Zn(2+), or Mn(2+). In the second group, almost half of the nematodes moved to diacetyl by crossing the barrier of Ag(+), Cu(2+), Cr(2+), or Cd(2+). In the third group, only a small number of nematodes moved to diacetyl by crossing the barrier of Pb(2+) or Hg(2+). Moreover, when nematodes encountered different metal ion barriers during migration toward diacetyl, the percentage of nematodes moving back and then turning and that of nematodes moving straight to diacetyl were very different. With the aid of examined assay systems, it was found that mutations of fsn-1 that encodes a F-box protein, and its target scd-2 that encodes a receptor tyrosine kinase, caused severe defects in integrative response, and the sensory integration defects of fsn-1 mutants were obviously inhibited by scd-2 mutation. CONCLUSION:Based on the nematode behaviors in examined assay systems, 3 groups of assay systems were obtained. The first group may be helpful in evaluating or identifying the very subtle deficits in sensory integration, and the third group may be useful for the final confirmation of sensory integration defects of mutants identified in the first or the second group of assay systems. Furthermore, the important association of sensory integration regulation with stabilization or destabilization of synaptic differentiation may exist in C. elegans. |
| 巻・号 | 27(2) |
| ページ | 69-82 |
| 公開日 | 2011-4-1 |
| DOI | 10.1007/s12264-011-1152-z |
| PMID | 21441968 |
| PMC | PMC5560346 |
| MeSH | Analysis of Variance Animals Animals, Genetically Modified Behavior, Animal Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Diacetyl / pharmacology Dose-Response Relationship, Drug Escape Reaction / drug effects Escape Reaction / physiology F-Box Proteins / genetics F-Box Proteins / metabolism Gene Expression Regulation / drug effects Green Fluorescent Proteins / genetics Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism Locomotion / drug effects Locomotion / genetics Metals, Heavy / pharmacology Mutation / genetics Neural Inhibition / drug effects Neural Inhibition / genetics Neural Inhibition / physiology* Olfactory Pathways / drug effects Olfactory Pathways / physiology* Protein-Tyrosine Kinases / deficiency Protein-Tyrosine Kinases / genetics Sensation / drug effects Sensation / genetics Sensation / physiology* Sensory Receptor Cells / physiology* Signal Transduction / drug effects Signal Transduction / genetics Stimulation, Chemical Visceral Afferents / drug effects Visceral Afferents / physiology* gamma-Aminobutyric Acid / metabolism |
| IF | 4.326 |
| 引用数 | 13 |
| WOS 分野 | NEUROSCIENCES |
| リソース情報 | |
| 線虫 | tm501 |