RRC ID |
46149
|
著者 |
Lee K, Shim J, Bae J, Kim YJ, Lee J.
|
タイトル |
Stabilization of RNT-1 protein, runt-related transcription factor (RUNX) protein homolog of Caenorhabditis elegans, by oxidative stress through mitogen-activated protein kinase pathway.
|
ジャーナル |
J Biol Chem
|
Abstract |
RUNX proteins are evolutionarily conserved transcription factors known to be involved in various developmental processes. Here we report a new role for a RUNX protein: a role in stress response. We show that RNT-1, the Caenorhabditis elegans RUNX homolog, is constantly produced and degraded by the ubiquitination-proteasome pathway in the intestine of the nematode. RNT-1 was rapidly stabilized by oxidative stress, and the rnt-1-mutant animals were more sensitive to oxidative stress, indicating that rapid RNT-1 stabilization is a defense response against the oxidative stress. The MAP kinase pathway is required for RNT-1 stabilization, and RNT-1 was phosphorylated by SEK-1/PMK-1 in vitro. ChIP-sequencing analysis revealed a feedback loop mechanism of the MAP kinase pathway by the VHP-1 phosphatase in the RNT-1-mediated oxidative stress response. We propose that rnt-1 is regulated at the protein level for its role in the immediate response to environmental challenges in the intestine.
|
巻・号 |
287(13)
|
ページ |
10444-10452
|
公開日 |
2012-3-23
|
DOI |
10.1074/jbc.M111.314146
|
PII |
S0021-9258(20)65454-0
|
PMID |
22308034
|
PMC |
PMC3323012
|
MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Core Binding Factor alpha Subunits
Dual-Specificity Phosphatases / genetics
Dual-Specificity Phosphatases / metabolism
Intestinal Mucosa / metabolism*
MAP Kinase Signaling System / physiology*
Oxidative Stress / physiology*
Phosphorylation / physiology
Protein Stability
Proteolysis*
Receptor, EphA4 / genetics
Receptor, EphA4 / metabolism
Sequence Homology, Amino Acid
Transcription Factors / genetics
Transcription Factors / metabolism*
Ubiquitination / physiology
|
IF |
4.238
|
引用数 |
11
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
線虫 |
tm388
tm491 |