RRC ID 46149
著者 Lee K, Shim J, Bae J, Kim YJ, Lee J.
タイトル Stabilization of RNT-1 protein, runt-related transcription factor (RUNX) protein homolog of Caenorhabditis elegans, by oxidative stress through mitogen-activated protein kinase pathway.
ジャーナル J Biol Chem
Abstract RUNX proteins are evolutionarily conserved transcription factors known to be involved in various developmental processes. Here we report a new role for a RUNX protein: a role in stress response. We show that RNT-1, the Caenorhabditis elegans RUNX homolog, is constantly produced and degraded by the ubiquitination-proteasome pathway in the intestine of the nematode. RNT-1 was rapidly stabilized by oxidative stress, and the rnt-1-mutant animals were more sensitive to oxidative stress, indicating that rapid RNT-1 stabilization is a defense response against the oxidative stress. The MAP kinase pathway is required for RNT-1 stabilization, and RNT-1 was phosphorylated by SEK-1/PMK-1 in vitro. ChIP-sequencing analysis revealed a feedback loop mechanism of the MAP kinase pathway by the VHP-1 phosphatase in the RNT-1-mediated oxidative stress response. We propose that rnt-1 is regulated at the protein level for its role in the immediate response to environmental challenges in the intestine.
巻・号 287(13)
ページ 10444-10452
公開日 2012-3-23
DOI 10.1074/jbc.M111.314146
PII S0021-9258(20)65454-0
PMID 22308034
PMC PMC3323012
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Core Binding Factor alpha Subunits Dual-Specificity Phosphatases / genetics Dual-Specificity Phosphatases / metabolism Intestinal Mucosa / metabolism* MAP Kinase Signaling System / physiology* Oxidative Stress / physiology* Phosphorylation / physiology Protein Stability Proteolysis* Receptor, EphA4 / genetics Receptor, EphA4 / metabolism Sequence Homology, Amino Acid Transcription Factors / genetics Transcription Factors / metabolism* Ubiquitination / physiology
IF 4.238
引用数 11
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm388 tm491