RRC ID |
46128
|
著者 |
Ihara S, Hagedorn EJ, Morrissey MA, Chi Q, Motegi F, Kramer JM, Sherwood DR.
|
タイトル |
Basement membrane sliding and targeted adhesion remodels tissue boundaries during uterine-vulval attachment in Caenorhabditis elegans.
|
ジャーナル |
Nat Cell Biol
|
Abstract |
Large gaps in basement membrane occur at sites of cell invasion and tissue remodelling in development and cancer. Though never followed directly in vivo, basement membrane dissolution or reduced synthesis have been postulated to create these gaps. Using landmark photobleaching and optical highlighting of laminin and type IV collagen, we find that a new mechanism, basement membrane sliding, underlies basement membrane gap enlargement during uterine-vulval attachment in Caenorhabditis elegans. Laser ablation and mutant analysis reveal that the invaginating vulval cells promote basement membrane movement. Further, an RNA interference and expression screen identifies the integrin INA-1/PAT-3 and VAB-19, homologue of the tumour suppressor Kank, as regulators of basement membrane opening. Both concentrate within vulval cells at the basement membrane gap boundary and halt expansion of the shifting basement membrane. Basement membrane sliding followed by targeted adhesion represents a new mechanism for creating precise basement membrane breaches that can be used by cells to break down compartment boundaries.
|
巻・号 |
13(6)
|
ページ |
641-51
|
公開日 |
2011-6-1
|
DOI |
10.1038/ncb2233
|
PII |
ncb2233
|
PMID |
21572423
|
PMC |
PMC3107347
|
MeSH |
Animals
Basement Membrane / physiology*
Basement Membrane / ultrastructure
Caenorhabditis elegans*
Cell Adhesion
Female
Uterus / physiology
Vulva / physiology
|
IF |
20.042
|
引用数 |
60
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
線虫 |
tm1291 |