論文 - 詳細
| RRC ID | 46086 |
|---|---|
| 著者 | Miki TS, Richter H, Rüegger S, Großhans H. |
| タイトル | PAXT-1 promotes XRN2 activity by stabilizing it through a conserved domain. |
| ジャーナル | Mol Cell |
| Abstract |
XRN2 is an essential eukaryotic exoribonuclease that processes and degrades various substrates. Here we identify the previously uncharacterized protein R05D11.6/PAXT-1 as a subunit of an XRN2 complex in C. elegans. Targeted paxt-1 inactivation through TALEN-mediated genome editing reduces XRN2 levels, decreases miRNA turnover activity, and results in worm death, which can be averted by overexpressing xrn-2. Hence, stabilization of XRN2 is a major function of PAXT-1. A truncated PAXT-1 protein retaining a predicted domain of unknown function (DUF3469) suffices to restore viability to paxt-1 mutant animals, elevates XRN2 levels, and binds to XRN2. This domain occurs in additional metazoan proteins and mediates interaction of human CDKN2AIP/CARF and NKRF/NRF with XRN2. Thus, we have identified a bona fide XRN2-binding domain (XTBD) that can link different proteins, and possibly functionalities, to XRN2. |
| 巻・号 | 53(2) |
| ページ | 351-60 |
| 公開日 | 2014-1-23 |
| DOI | 10.1016/j.molcel.2014.01.001 |
| PII | S1097-2765(14)00003-3 |
| PMID | 24462208 |
| MeSH | Animals Apoptosis Regulatory Proteins / metabolism Binding Sites Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / chemistry Carrier Proteins / genetics Carrier Proteins / metabolism* Conserved Sequence DNA-Binding Proteins Exoribonucleases / metabolism Gene Knockout Techniques Humans Protein Structure, Tertiary RNA Stability RNA-Binding Proteins / metabolism Repressor Proteins / metabolism Transcription Factors / metabolism |
| IF | 15.584 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm3473 |