RRC ID 46042
著者 Castro C, Sar F, Shaw WR, Mishima M, Miska EA, Griffin JL.
タイトル A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in Caenorhabditis elegans.
ジャーナル BMC Genomics
Abstract BACKGROUND:Caenorhabditis elegans provides a genetically tractable model organism to investigate the network of genes involved in fat metabolism and how regulation is perturbed to produce the complex phenotype of obesity. C. elegans possess the full range of desaturases, including the Δ9 desaturases expressed by fat-5, fat-6 and fat-7. They regulate the biosynthesis of monounsaturated fatty acids, used for the synthesis of lipids including phospholipids, triglycerides and cholesteryl esters.
RESULTS:Liquid chromatography mass spectrometry (LC-MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy were used to define the metabolome of all the possible knock-outs for the Δ9 desaturases, including for the first time intact lipids. Despite the genes having similar enzymatic roles, excellent discrimination was achievable for all single and viable double mutants highlighting the distinctive roles of fat-6 and fat-7, both expressing steroyl-CoA desaturases. The metabolomic changes extend to aqueous metabolites demonstrating the influence Δ9 desaturases have on regulating global metabolism and highlighting how comprehensive metabolomics is more discriminatory than classically used dyes for fat staining.
CONCLUSIONS:The propagation of metabolic changes across the network of metabolism demonstrates that modification of the Δ9 desaturases places C.elegans into a catabolic state compared with wildtype controls.
巻・号 13
ページ 36
公開日 2012-1-20
DOI 10.1186/1471-2164-13-36
PII 1471-2164-13-36
PMID 22264337
PMC PMC3398271
MeSH Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / enzymology Caenorhabditis elegans / metabolism* Chromatography, High Pressure Liquid Fatty Acids / metabolism Gas Chromatography-Mass Spectrometry Gene Knockout Techniques Lipids / analysis* Magnetic Resonance Spectroscopy Mass Spectrometry Metabolome* Stearoyl-CoA Desaturase / antagonists & inhibitors Stearoyl-CoA Desaturase / genetics Stearoyl-CoA Desaturase / metabolism*
IF 3.594
引用数 16
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
線虫 tm331 tm420