RRC ID 46039
著者 Large EE, Mathies LD.
タイトル Caenorhabditis elegans SWI/SNF subunits control sequential developmental stages in the somatic gonad.
ジャーナル G3 (Bethesda)
Abstract The Caenorhabditis elegans somatic gonadal precursors (SGPs) are multipotent progenitors that give rise to all somatic tissues of the adult reproductive system. The hunchback and Ikaros-like gene ehn-3 is expressed specifically in SGPs and is required for their development into differentiated tissues of the somatic gonad. To find novel genes involved in SGP development, we used a weak allele of ehn-3 as the basis for a reverse genetic screen. Feeding RNAi was used to screen ∼2400 clones consisting of transcription factors, signaling components, and chromatin factors. The screen identified five members of the C. elegans SWI/SNF chromatin remodeling complex as genetic enhancers of ehn-3. We characterized alleles of 10 SWI/SNF genes and found that SWI/SNF subunits are required for viability and gonadogenesis. Two conserved SWI/SNF complexes, PBAF and BAF, are defined by their unique array of accessory subunits around a common enzymatic core that includes a catalytic Swi2/Snf2-type ATPase. Tissue-specific RNAi experiments suggest that C. elegans PBAF and BAF complexes control different processes during somatic gonadal development: PBRM-1, a signature subunit of PBAF, is important for normal SGP development, whereas LET-526, the distinguishing subunit of BAF, is required for development of a differentiated cell type, the distal tip cell (DTC). We found that the SWSN-4 ATPase subunit is required for SGP and DTC development. Finally, we provide evidence that C. elegans PBAF subunits and hnd-1/dHand are important for the cell fate decision between SGPs and their differentiated sisters, the head mesodermal cells.
巻・号 4(3)
ページ 471-83
公開日 2014-3-20
DOI 10.1534/g3.113.009852
PII g3.113.009852
PMID 24402584
PMC PMC3962486
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gonads / growth & development* Mesoderm / metabolism Phenotype RNA Interference Transcription Factors / genetics Transcription Factors / metabolism*
IF 2.781
引用数 7
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm415 tm4795 tm3395 tm4263 tm3309 tm3647 tm305 tm4567 tm2127