RRC ID 46012
著者 Ko S, Kawasaki I, Shim YH.
タイトル PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1.
ジャーナル PLoS One
Abstract Poly(A)-binding proteins are highly conserved among eukaryotes and regulate stability of mRNA and translation. Among C. elegans homologues, pab-1 mutants showed defects in germline mitotic proliferation. Unlike pab-1 mutants, pab-1 RNAi at every larval stage caused arrest of germline development at the following stage, indicating that pab-1 is required for the entire postembryonic germline development. This idea is supported by the observations that the mRNA level of pab-1 increased throughout postembryonic development and its protein expression was germline-enriched. PAB-1 localized to P granules and the cytoplasm in the germline. PAB-1 colocalized with CGH-1 and CAR-1 and affected their localization, suggesting that PAB-1 is a component of processing (P)-bodies that interacts with them. The mRNA and protein levels of representative germline genes, rec-8, GLP-1, rme-2, and msp-152, were decreased after pab-1 RNAi. Although the mRNA level of msp-152 was increased in cgh-1 mutant, it was also significantly reduced by pab-1 RNAi. Our results suggest that PAB-1 positively regulates the mRNA levels of germline genes, which is likely facilitated by the interaction of PAB-1 with other P-body components, CGH-1 and CAR-1.
巻・号 8(12)
ページ e84798
公開日 2013-1-1
DOI 10.1371/journal.pone.0084798
PII PONE-D-13-36282
PMID 24367695
PMC PMC3868610
MeSH Animals Blotting, Western Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / metabolism* DNA Primers / genetics Fluorescent Antibody Technique Gene Expression Regulation, Developmental / physiology* Germ Cells / metabolism* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Poly(A)-Binding Protein I / genetics Poly(A)-Binding Protein I / metabolism* RNA Interference RNA Nucleotidyltransferases / metabolism* RNA, Messenger / metabolism* RNA-Binding Proteins / metabolism* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction
IF 2.74
引用数 6
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1753