RRC ID 46006
著者 Kershner AM, Shin H, Hansen TJ, Kimble J.
タイトル Discovery of two GLP-1/Notch target genes that account for the role of GLP-1/Notch signaling in stem cell maintenance.
ジャーナル Proc Natl Acad Sci U S A
Abstract A stem cell's immediate microenvironment creates an essential "niche" to maintain stem cell self-renewal. Many niches and their intercellular signaling pathways are known, but for the most part, the key downstream targets of niche signaling remain elusive. Here, we report the discovery of two GLP-1/Notch target genes, lst-1 (lateral signaling target) and sygl-1 (synthetic Glp), that function redundantly to maintain germ-line stem cells (GSCs) in the nematode Caenorhabditis elegans. Whereas lst-1 and sygl-1 single mutants appear normal, lst-1 sygl-1 double mutants are phenotypically indistinguishable from glp-1/Notch mutants. Multiple lines of evidence demonstrate that GLP-1/Notch signaling activates lst-1 and sygl-1 expression in GSCs within the niche. Therefore, these two genes fully account for the role of GLP-1/Notch signaling in GSC maintenance. Importantly, lst-1 and sygl-1 are not required for GLP-1/Notch signaling per se. We conclude that lst-1 and sygl-1 forge a critical link between Notch signaling and GSC maintenance.
巻・号 111(10)
ページ 3739-44
公開日 2014-3-11
DOI 10.1073/pnas.1401861111
PII 1401861111
PMID 24567412
PMC PMC3956202
MeSH Benzimidazoles Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation / genetics Gene Expression Regulation / physiology Glucagon-Like Peptide 1 / genetics Glucagon-Like Peptide 1 / metabolism* Immunohistochemistry In Situ Hybridization Polysorbates RNA Interference Receptors, Notch / metabolism* Signal Transduction / physiology* Stem Cells / physiology*
IF 9.412
引用数 38
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm5040