| RRC ID |
45988
|
| 著者 |
Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A.
|
| タイトル |
DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Developmental timing genes catalyze stem cell progression and animal maturation programs across taxa. Caenorhabditis elegans DRE-1/FBXO11 functions in an SCF E3-ubiquitin ligase complex to regulate the transition to adult programs, but its cognate proteolytic substrates are unknown. Here, we identify the conserved transcription factor BLMP-1 as a substrate of the SCF(DRE-1/FBXO11) complex. blmp-1 deletion suppressed dre-1 mutant phenotypes and exhibited developmental timing defects opposite to dre-1. blmp-1 also opposed dre-1 for other life history traits, including entry into the dauer diapause and longevity. BLMP-1 protein was strikingly elevated upon dre-1 depletion and dysregulated in a stage- and tissue-specific manner. The role of DRE-1 in regulating BLMP-1 stability is evolutionary conserved, as we observed direct protein interaction and degradation function for worm and human counterparts. Taken together, posttranslational regulation of BLMP-1/BLIMP-1 by DRE-1/FBXO11 coordinates C. elegans developmental timing and other life history traits, suggesting that this two-protein module mediates metazoan maturation processes.
|
| 巻・号 |
28(6)
|
| ページ |
697-710
|
| 公開日 |
2014-3-31
|
| DOI |
10.1016/j.devcel.2014.01.028
|
| PII |
S1534-5807(14)00069-0
|
| PMID |
24613396
|
| PMC |
PMC4040421
|
| MeSH |
Animals
Blotting, Western
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / antagonists & inhibitors
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cell Differentiation
Epidermal Cells
Epidermis / metabolism
F-Box Proteins / antagonists & inhibitors
F-Box Proteins / genetics
F-Box Proteins / metabolism*
Gene Expression Regulation, Developmental*
Gene Silencing
Gonads / cytology
Gonads / metabolism
HEK293 Cells
Humans
Immunoenzyme Techniques
Larva / cytology*
Larva / metabolism
Longevity / genetics*
Organ Specificity
Proteasome Endopeptidase Complex / metabolism
Proteolysis
RNA, Small Interfering / genetics
Time Factors
Transcription Factors / genetics
Transcription Factors / metabolism
Ubiquitination
|
| IF |
10.092
|
| 引用数 |
30
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm548 |
