RRC ID 45916
著者 Wu Y, Cheng S, Zhao H, Zou W, Yoshina S, Mitani S, Zhang H, Wang X.
タイトル PI3P phosphatase activity is required for autophagosome maturation and autolysosome formation.
ジャーナル EMBO Rep
Abstract Autophagosome formation is promoted by the PI3 kinase complex and negatively regulated by myotubularin phosphatases, indicating that regulation of local phosphatidylinositol 3-phosphate (PtdIns3P) levels is important for this early phase of autophagy. Here, we show that the Caenorhabditis elegans myotubularin phosphatase MTM-3 catalyzes PtdIns3P turnover late in autophagy. MTM-3 acts downstream of the ATG-2/EPG-6 complex and upstream of EPG-5 to promote autophagosome maturation into autolysosomes. MTM-3 is recruited to autophagosomes by PtdIns3P, and loss of MTM-3 causes increased autophagic association of ATG-18 in a PtdIns3P-dependent manner. Our data reveal critical roles of PtdIns3P turnover in autophagosome maturation and/or autolysosome formation.
巻・号 15(9)
ページ 973-81
公開日 2014-9-1
DOI 10.15252/embr.201438618
PII embr.201438618
PMID 25124690
PMC PMC4198041
MeSH Animals Autophagy / genetics* Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism Lysosomes / genetics Lysosomes / metabolism Phagosomes / genetics* Phosphatidylinositol 3-Kinases / genetics Phosphatidylinositol 3-Kinases / metabolism* Phosphatidylinositol Phosphates / metabolism* Phosphoric Monoester Hydrolases / metabolism Protein Tyrosine Phosphatases, Non-Receptor / metabolism
IF 7.497
引用数 26
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm4475