| RRC ID |
45893
|
| 著者 |
Yang H, Chen YZ, Zhang Y, Wang X, Zhao X, Godfroy JI 3rd, Liang Q, Zhang M, Zhang T, Yuan Q, Ann Royal M, Driscoll M, Xia NS, Yin H, Xue D.
|
| タイトル |
A lysine-rich motif in the phosphatidylserine receptor PSR-1 mediates recognition and removal of apoptotic cells.
|
| ジャーナル |
Nat Commun
|
| Abstract |
The conserved phosphatidylserine receptor (PSR) was first identified as a receptor for phosphatidylserine, an 'eat-me' signal exposed by apoptotic cells. However, several studies suggest that PSR may also act as an arginine demethylase, a lysyl hydroxylase, or an RNA-binding protein through its N-terminal JmjC domain. How PSR might execute drastically different biochemical activities, and whether they are physiologically significant, remain unclear. Here we report that a lysine-rich motif in the extracellular domain of PSR-1, the Caenorhabditis elegans PSR, mediates specific phosphatidylserine binding in vitro and clearance of apoptotic cells in vivo. This motif also mediates phosphatidylserine-induced oligomerization of PSR-1, suggesting a mechanism by which PSR-1 activates phagocytosis. Mutations in the phosphatidylserine-binding motif, but not in its Fe(II) binding site critical for the JmjC activity, abolish PSR-1 phagocytic function. Moreover, PSR-1 enriches and clusters around apoptotic cells during apoptosis. These results establish that PSR-1 is a conserved, phosphatidylserine-recognizing phagocyte receptor.
|
| 巻・号 |
6
|
| ページ |
5717
|
| 公開日 |
2015-1-7
|
| DOI |
10.1038/ncomms6717
|
| PII |
ncomms6717
|
| PMID |
25564762
|
| PMC |
PMC4306451
|
| MeSH |
Amino Acid Motifs / genetics*
Amino Acid Sequence
Animals
Animals, Genetically Modified
Apoptosis
Base Sequence
Blotting, Western
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / chemistry*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cloning, Molecular
DNA Primers / genetics
Enzyme-Linked Immunosorbent Assay
Fluorescence
Germ Cells
Green Fluorescent Proteins
Immunoprecipitation
Models, Molecular*
Molecular Sequence Data
Polymerase Chain Reaction
Receptors, Cell Surface / chemistry*
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Sequence Analysis, DNA
Time-Lapse Imaging
Ultraviolet Rays
|
| IF |
12.121
|
| 引用数 |
24
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm469 |
